Identification of potential key genes associated with severe pneumonia using mRNA‑seq

Feng,Cong; Huang,He; Huang,Sai; Zhai,Yong‑Zhi; Dong,Jing; Chen,Li; Huang,Zhi; Zhou,Xuan; Li,Bei; Wang,Li‑Li; Chen,Wei; Lv,Fa‑Qin; Li,Tan‑Shi

doi:10.3892/etm.2018.6262

Identification of potential key genes associated with severe pneumonia using mRNA‑seq

Authors:
- Cong Feng
- He Huang
- Sai Huang
- Yong‑Zhi Zhai
- Jing Dong
- Li Chen
- Zhi Huang
- Xuan Zhou
- Bei Li
- Li‑Li Wang
- Wei Chen
- Fa‑Qin Lv
- Tan‑Shi Li
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Affiliations: Department of Emergency, Chinese PLA General Hospital, Beijing 100853, P.R. China, Department of Critical Care Medicine, General Hospital of Jinan Command, Jinan, Shandong 250031, P.R. China, Department of Electrical and Computer Engineering, Purdue University, Indianapolis, IN 46202, USA, Department of Ultrasound, Chinese PLA General Hospital, Beijing 100853, P.R. China
Published online on: June 7, 2018 https://doi.org/10.3892/etm.2018.6262
Pages: 758-766
Copyright: © Feng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

This study aimed to identify the potential key genes associated with severe pneumonia using mRNA‑seq. Nine peripheral blood samples from patients with severe pneumonia alone (SP group, n=3) and severe pneumonia accompanied with chronic obstructive pulmonary disease (COPD; CSP group, n=3), as well as volunteers without pneumonia (control group, n=3) underwent mRNA‑seq. Based on the sequencing data, differentially expressed genes (DEGs) were identified by Limma package. Following the pathway enrichment analysis of DEGs, the genes that were differentially expressed in the SP and CSP groups were selected for pathway enrichment analysis and coexpression analysis. In addition, potential genes related to pneumonia were identified based on the information in the Comparative Toxicogenomics Database. In total, 645 and 528 DEGs were identified in the SP and CSP groups, respectively, compared with the normal controls. Among these DEGs, 88 upregulated genes and 80 downregulated genes were common between the two groups. The functions of the common DEGs were similar to those of the DEGs in the SP group. In the coexpression network, the commonly downregulated genes (including ND1, ND3, ND4L, and ND6) and the commonly upregulated genes (including TSPY6P and CDY10P) exhibited a higher degree. In addition, 131 DEGs (including ND1, ND3, ND6, MIR449A and TAS2R43) were predicted to be potential pneumonia‑related genes. In conclusion, the present study demonstrated that the common DEGs may be associated with the progression of severe pneumonia.

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