Open Access

Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing

  • Authors:
    • Huang‑De Fu
    • Shu Wang
    • Bin Ge
    • Li‑Qing Li
    • Hong‑Meng Zeng
    • Qing‑Feng Shu
    • Yang Zhou
  • View Affiliations

  • Published online on: July 2, 2018     https://doi.org/10.3892/etm.2018.6390
  • Pages: 1987-1993
  • Copyright: © Fu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Moist exposed burn ointment (MEBO) is becoming increasingly popular in China as it shortens wound‑healing time and reduces scar formation. However, its exact mechanism in mediating the wound‑healing process is not yet clear. In the present study a total of 90 healthy adult male Wistar rats of specific-pathogen-free grade were divided equally into a control group, wound group, MEBO group, recombinant bovine basic fibroblast growth factor (rb‑bFGF) group and sham operation group. Wound healing was observed from the extracted granulation tissues and recorded at three time points on 3, 7 and 14 days. Different levels of tumor necrosis factor α (TNF‑α) and interleukin‑6 (IL‑6) in tissue homogenate were detected using ELISA. Western blot analysis and quantitative PCR were used to detect the expression of nerve growth factor (NGF), substance P (SP) as well as tyrosine kinase A (TrkA) receptor protein and the corresponding mRNA levels in granulation tissue. It was observed that the wound healing progressed faster in the MEBO and rb‑bFGF groups compared with the wound group (P<0.01). TNF‑α and IL‑6 had an upward‑downward trend at three time points, with the wound group demonstrating the most obvious increase (P<0.01). NGF and SP mRNA and protein levels in granulation tissue in MEBO, rb‑bFGF and sham operation groups reached their highest levels on day 7 and then decreased on day 14. The expression level of TrkA was also measured simultaneously and its expression pattern was similar to that of NGF and SP. These results suggested that MEBO may promote nerve repair and accelerate wound healing through mediating the expression levels of NGF and SP, as well as TrkA.

References

1 

Huang Y, Qi SH, Shu B, Mao RX, Xie JL and Xu YB: In vivo study on the effect of Skin-derived precursors (SKPs) on neural repair in skin wounds. Chin J Nerv Mental Dis. 37:531–534. 2011.(In Chinese).

2 

Sulaiman W and Dreesen TD: Effect of local application of transforming growth factor-β at the nerve repair site following chronic axotomy and denervation on the expression of regeneration-associated genes. Laboratory investigation. J Neurosurg. 121:859–874. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Xu RQ: Theory and practice of regenerative medicine: A speech at the 7th National Academic conference on Burns and Wounds. Chin J Burns Wound Surf Ulcers. 14:201–215. 2002.(In Chinese).

4 

Mabrouk A, Boughdadi NS, Helal HA, Zaki BM and Maher A: Moist occlusive dressing (Aquacel(®) Ag) versus moist open dressing (MEBO(®)) in the management of partial-thickness facial burns: A comparative study in Ain Shams University. Burns. 38:396–403. 2012. View Article : Google Scholar : PubMed/NCBI

5 

Tang QL, Han SS, Feng J, Di JQ, Qin WX, Fu J and Jiang QY: Moist exposed burn ointment promotes cutaneous excisional wound healing in rats involving VEGF and bFGF. Mol Med Rep. 9:1277–1282. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Tang QL, Huang X and Wang Y: Ultrastructural Pathology and the Mechanism of Expression of MAPKK mRNA & c-myc mRNA of Chronic Refractory Wound Treated by MEBT/MEBO. Chin Gen Pract. 18:294–299. 2015.(In Chinese).

7 

Li NQ: New Century National College of Integrative Medicine clinical medicine professional planning teaching materials ‘integrated traditional Chinese and Western medicine’. China Traditional Chinese Medicine Press; Bejing: 2005

8 

He QH: National traditional chinese medicine industry higher education ‘12th five-year’ national planning textbook ‘integrative medicinesurgery’. 2nd edition. China Traditional Chinese Medicine Press; Bejing: 2014

9 

He QH: National traditional chinese medicine industry higher education ‘13th five-year’ national planning textbook ‘integrative medicine surgery’. 3rd edition. China Traditional Chinese Medicine Press; Bejing: 2016

10 

Xu RQ and Xiao M: The mechanism of burn regenerative therapy and wound healing. Chin J Burns Wounds Surface Ulcers. 15:253–261. 2003.(In Chinese).

11 

El-Hadidy MR, El-Hadidy AR, Bhaa A, Asker SA and Mazroa SA: Role of epidermal stem cells in repair of partial-thickness burn injury after using moist exposed burn ointment (MEBO(®)) histological and immunohistochemical study. Tissue Cell. 46:144–151. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Muir D: The potentiation of peripheral nerve sheaths in regeneration and repair. Exp Neurol. 223:102–111. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Griffin JW, Hogan MV, Chhabra AB and Deal DN: Peripheral nerve repair and reconstruction. J Bone Joint Surg Am. 95:2144–2151. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Schecterson LC and Bothwell M: Neurotrophin receptors: Old friends with new partners. Dev Neurobiol. 70:332–338. 2010.PubMed/NCBI

15 

Xu YF: Effects of FGF19-FGFR4 and NGF-TrkA Signaling Pathways on Progression and Prognosis of Cholangiocarcinoma. PhD dissertationShandong University 2014

16 

Yang XQ: The preliminary study of expression and interaction of EGFR family and NGF/TrkA in cholangiocarcinoma. PhD dissertationShandong University 2014

17 

Ji YX: Expression of Nerve Growth Factor in Diabetic Foot Wound Tissue. PhD dissertationHuazhong University of Science and Technology 2011

18 

Fowler JR, Lavasani M, Huard J and Goitz RJ: Biologic strategies to improve nerve regeneration after peripheral nerve repair. J Reconstr Microsurg. 31:243–248. 2015.PubMed/NCBI

19 

Hosli E and Hosli L: Receptors for neurotransmitters on astrocytes in the mammalian central nervous system. Prog Neurobiol. 40:477–506. 1993. View Article : Google Scholar : PubMed/NCBI

20 

Hamke M, Herpfer I, Lieb K, Wandelt C and Fiebich BL: Substance P induces expression of the corticotropin-releasing factor receptor 1 by activation of the neurokinin-1 receptor. Brain Res. 1102:135–144. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Xu G: Investigation on the Interaction Mechanism of Nerve Growth Factor NGF and Neuropeptide SP in Hypertrophic Scar. PhD dissertationSouth China University 2015

22 

Jia Y: The relationship between exogenous substance p and focal adhesion kinase, protein kinase B and nuclear factor in scar formation. Shanxi Medical University; 2012

23 

Zhao JY, Fu XB and Lei YH: Preparation of small area whole layer skin defect wound model in rats. Infect Inflamm Rep. 9:642008.

24 

Shen DX: A Quantitative Method for the Study of Anti-inflammatory Effects of Chinese and Western Medicine. Chin J IntegTradit West Med. 3:491983.(In Chinese).

25 

Tang QL: Burn skin regenerative medical technology clinical application specification (Chinese Edition). Chin Trad Chinese Med Press; Beijing: 2013

26 

Ma FS: Accumulative eschar after burn. Clin Case Rep. 4:151–153. 2015. View Article : Google Scholar : PubMed/NCBI

27 

Sakallioglu AE, Basaran O, Karakayali H, Ozdemir BH, Yucel M, Arat Z and Haberal M: Interactions of systemic immune response and local wound healing in different burn depths: An experimental study on rats. J Burn Care Res. 27:357–366. 2006. View Article : Google Scholar : PubMed/NCBI

Related Articles

Journal Cover

September 2018
Volume 16 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Fu, H., Wang, S., Ge, B., Li, L., Zeng, H., Shu, Q., & Zhou, Y. (2018). Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing. Experimental and Therapeutic Medicine, 16, 1987-1993. https://doi.org/10.3892/etm.2018.6390
MLA
Fu, H., Wang, S., Ge, B., Li, L., Zeng, H., Shu, Q., Zhou, Y."Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing". Experimental and Therapeutic Medicine 16.3 (2018): 1987-1993.
Chicago
Fu, H., Wang, S., Ge, B., Li, L., Zeng, H., Shu, Q., Zhou, Y."Nerve growth factor and substance P may be involved in moist exposed burn ointment‑mediated chronic refractory wound healing". Experimental and Therapeutic Medicine 16, no. 3 (2018): 1987-1993. https://doi.org/10.3892/etm.2018.6390