Open Access

Dendritic cell vaccine with Ag85A enhances anti‑colorectal carcinoma immunity

  • Authors:
    • Jingbo Zhai
    • Wei Gao
    • Leheng Zhao
    • Zhipeng Gao
    • Xuefeng Jiang
    • Changlong Lu
  • View Affiliations

  • Published online on: October 11, 2018     https://doi.org/10.3892/etm.2018.6851
  • Pages: 5123-5129
  • Copyright: © Zhai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dendritic cells (DCs) are able to trigger T‑cell activation and thus have been considered important for vaccine production against cancers. Vaccines containing DCs have been reported to be effective for developing immunity against cancer cells. The interactions between DCs and auxiliary agents are critical in the development of second‑generation vaccines. In the present study, it was evaluated whether Ag85A‑mixed DCs could enhance anti‑tumor immunity in laboratory mice with colorectal carcinoma. Functional and phenotypic analyses of the effects of Ag85A‑mixed DCs were conducted via flow cytometry and measurement of T‑cell proliferation. In addition, interferon (IFN)‑γ production was assessed. The therapeutic efficacy of DC vaccination for colorectal carcinoma treatment in mice was investigated. It was identified that Ag85A‑mixed DCs exhibited strong upregulation of CD80, CD86 and major histocompatibility complex class II. Cytotoxic T‑lymphocytes with CT26‑primed Ag85A‑DCs were indicated to induce stronger responses against CT26 tumor cells and trigger IFN‑γ production. Furthermore, the Ag85A‑mixed DC vaccine exerted a considerable inhibitory effect on tumor progression in mice as compared with the control group. Therefore, DCs in combination with the Ag85A gene may reinforce anti‑colorectal carcinoma immunity. The current study provides a novel potential strategy for cancer treatment by enhancing immunity via Ag85A‑mixed DC vaccination.
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December-2018
Volume 16 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
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Spandidos Publications style
Zhai J, Gao W, Zhao L, Gao Z, Jiang X and Lu C: Dendritic cell vaccine with Ag85A enhances anti‑colorectal carcinoma immunity. Exp Ther Med 16: 5123-5129, 2018
APA
Zhai, J., Gao, W., Zhao, L., Gao, Z., Jiang, X., & Lu, C. (2018). Dendritic cell vaccine with Ag85A enhances anti‑colorectal carcinoma immunity. Experimental and Therapeutic Medicine, 16, 5123-5129. https://doi.org/10.3892/etm.2018.6851
MLA
Zhai, J., Gao, W., Zhao, L., Gao, Z., Jiang, X., Lu, C."Dendritic cell vaccine with Ag85A enhances anti‑colorectal carcinoma immunity". Experimental and Therapeutic Medicine 16.6 (2018): 5123-5129.
Chicago
Zhai, J., Gao, W., Zhao, L., Gao, Z., Jiang, X., Lu, C."Dendritic cell vaccine with Ag85A enhances anti‑colorectal carcinoma immunity". Experimental and Therapeutic Medicine 16, no. 6 (2018): 5123-5129. https://doi.org/10.3892/etm.2018.6851