Open Access

Decoy receptor‑3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease

  • Authors:
    • Xinjing Chen
    • Rehua Wang
    • Wei Chen
    • Li Lai
    • Zhiliang Li
  • View Affiliations

  • Published online on: January 30, 2019     https://doi.org/10.3892/etm.2019.7222
  • Pages: 2614-2622
  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Coronary heart disease is a disease characterized by coronary artery atherosclerosis lesions caused by vascular cavity stenosis, occlusion, myocardial ischemia, hypoxia or necrosis. Previous studies have demonstrated that decoy receptor‑3 (DCR‑3) can act as a pleiotropic immunomodulation for enhancing angiogenesis, which may be associated with the progression of coronary heart disease. In the present study, ELISA assay was used to investigate the plasma concentration level of DCR‑3 in patients with coronary heart disease. The mRNA and protein level of DCR‑3 in myocardial cells were determined by reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. The role and molecular mechanism of DCR‑3 was also evaluated in myocardial cells in mice with coronary heart disease. The role of small interfering RNA that targeted phosphoinositide 3‑kinase (PI3K) in DCR‑3 mediated apoptosis was confirmed by terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling and immunofluorescence. C57BL/6 mice with coronary heart disease were used to evaluate the efficacy of DCR‑3 on inflammation and apoptosis. The data indicated that plasma concentration level of DCR‑3 was downregulated in mice with coronary heart disease and that DCR‑3 administration improved symptoms of coronary heart disease and prolonged survival of mice with coronary heart disease. In addition, it was demonstrated that DCR‑3 treatment suppressed the inflammatory response and apoptosis of myocardial cells. Circulating DCR‑3 concentration levels may be identified as a predictor of coronary heart disease and prognosis of coronary heart disease. Notably, it was also demonstrated that DCR‑3 inhibited inflammatory factor expression levels by regulation of the PI3K/protein kinase B (AKT) signaling pathway. Taken together, these results indicate that increasing circulating DCR‑3 plasma concentration is associated with degree of coronary heart disease, suggesting that DCR‑3 may be a promising drug for the treatment of coronary heart disease via regulating inflammation and apoptosis through the PI3K/AKT signaling pathway.
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April-2019
Volume 17 Issue 4

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Spandidos Publications style
Chen X, Wang R, Chen W, Lai L and Li Z: Decoy receptor‑3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease. Exp Ther Med 17: 2614-2622, 2019
APA
Chen, X., Wang, R., Chen, W., Lai, L., & Li, Z. (2019). Decoy receptor‑3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease. Experimental and Therapeutic Medicine, 17, 2614-2622. https://doi.org/10.3892/etm.2019.7222
MLA
Chen, X., Wang, R., Chen, W., Lai, L., Li, Z."Decoy receptor‑3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease". Experimental and Therapeutic Medicine 17.4 (2019): 2614-2622.
Chicago
Chen, X., Wang, R., Chen, W., Lai, L., Li, Z."Decoy receptor‑3 regulates inflammation and apoptosis via PI3K/AKT signaling pathway in coronary heart disease". Experimental and Therapeutic Medicine 17, no. 4 (2019): 2614-2622. https://doi.org/10.3892/etm.2019.7222