Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers

  • Authors:
    • Marianne Leirdal
    • Mohsen Shadidy
    • Øystein Røsok
    • Mouldy Sioud
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/ijmm.14.2.217
  • Pages: 217-222
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Abstract

The identification of differentially expressed genes in tumour cells should have important implications in understanding carcinogenesis and developing new therapeutic and prognostic biomarkers. We have combined PCR-based cDNA subtraction and Northern blotting to identify truly differentially expressed genes in breast cancer cell line SKBR3 as compared to normal human mammary epithelial cells (HMEC). Hybridizing probe molecules were rescued from the Hybond N+ membranes and then PCR reamplified. The PCR reamplification is possible due to the fact that all probe molecules contain the same pair of adapter sequences on both ends. After cloning and sequencing three known genes, ribosomal protein L19 (RPL19), ADP/ATP carrier protein and ErbB-2 with high-elevated mRNA levels in SKBR3 were identified. In addition, two overexpressed genes with unknown functions, CXYorf1-related protein and hypothetical protein PRO2605, were found. High-titer andibodies against the recombinant RPL19 were detected in 5 patients out of 50 patients investigated. Thus, the present novel strategy based on the combination of PCR-based cDNA subtraction and Northern blotting should facilitate the identification of truly differentially expressed biomarkers, which may offer the potential to determine the proper drug for an individual patient at a given stage of disease or treatment.

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August 2004
Volume 14 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Leirdal M, Shadidy M, Røsok Ø and Sioud M: Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers. Int J Mol Med 14: 217-222, 2004
APA
Leirdal, M., Shadidy, M., Røsok, Ø., & Sioud, M. (2004). Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers. International Journal of Molecular Medicine, 14, 217-222. https://doi.org/10.3892/ijmm.14.2.217
MLA
Leirdal, M., Shadidy, M., Røsok, Ø., Sioud, M."Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers". International Journal of Molecular Medicine 14.2 (2004): 217-222.
Chicago
Leirdal, M., Shadidy, M., Røsok, Ø., Sioud, M."Identification of genes differentially expressed in breast cancer cell line SKBR3: Potential identification of new prognostic biomarkers". International Journal of Molecular Medicine 14, no. 2 (2004): 217-222. https://doi.org/10.3892/ijmm.14.2.217