Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription

  • Authors:
    • Birger Dieriks
    • Patrick Van Oostveldt
  • View Affiliations

  • Published online on: March 13, 2012     https://doi.org/10.3892/ijmm.2012.937
  • Pages: 1031-1038
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Abstract

Cyclophilin B (CypB) is an ubiquitously expressed protein, which performs several intra- and extracellular functions. Despite its abundant use as a household protein, little is known about its exact cellular localization and dynamics. In the present study we show that endogenous CypB localizes in one of two distinct compartments, either within the endoplasmic reticulum (ER) or inside the nucleus, accumulating in the fibrillar centers of the nucleoli. By means of a genetic deletion screen, we identified a minimal nucleolar localization signal for efficient relocation to the nucleoli. Within the fibrillar centers, CypB colocalized with RNA polymerase, upstream binding factor-1 (UBF), fibrillarin and dyskerin (DCK1). Even after chemical disruption of the nucleoli, a strong interaction with these proteins remained. Using live cell imaging, we showed a persistent colocalization of CypB with proteins involved in the ribosome biogenesis during the transcriptionally more active phases of the cell cycle. Supported by in silico data, our observations suggest that CypB interacts with these proteins and is involved in ribosome biogenesis and RNA transcription.

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June 2012
Volume 29 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Dieriks, B., & Dieriks, B. (2012). Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription. International Journal of Molecular Medicine, 29, 1031-1038. https://doi.org/10.3892/ijmm.2012.937
MLA
Dieriks, B., Van Oostveldt, P."Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription". International Journal of Molecular Medicine 29.6 (2012): 1031-1038.
Chicago
Dieriks, B., Van Oostveldt, P."Spatiotemporal behavior of nuclear cyclophilin B indicates a role in RNA transcription". International Journal of Molecular Medicine 29, no. 6 (2012): 1031-1038. https://doi.org/10.3892/ijmm.2012.937