DMP1 is a target of let-7 in dental pulp cells

Yue,Jing; Wu,Buling; Gao,Jie; Huang,Xin; Li,Changxia; Ma,Dandan; Fang,Fuchun

doi:10.3892/ijmm.2012.982

DMP1 is a target of let-7 in dental pulp cells

Authors:
- Jing Yue
- Buling Wu
- Jie Gao
- Xin Huang
- Changxia Li
- Dandan Ma
- Fuchun Fang
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Affiliations: Department of Stomatology, Nanfang Hospital, College of Stomatology, Southern Medical University, Guangzhou, P.R. China
Published online on: April 25, 2012 https://doi.org/10.3892/ijmm.2012.982
Pages: 295-301

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Abstract

Members of the let-7 family have been shown to play a critical role in cell differentiation and tumorigenesis. However, potential targets of let-7 are still unclear. In the current study, we used bioinformatic analysis combined with DNA sequence analysis to identify potential let-7 targets. We discovered that dentin matrix protein 1 (DMP1), which is a non-collagenous protein essential in the mineralization of dentin and bone, has a let-7 binding site in its 3'-untranslated region. Furthermore, reporter assays demonstrated that the DMP1 3'-untranslated region can be regulated directly by the members of let-7. Gene expression levels of let-7 and DMP1 were validated by qRT-PCR of dental pulp cells cultured in a mineralizing medium. Our results suggest that DMP1 is regulated post-transcriptionally by let-7 during odontoblast differentiation.

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1.	GT HuangS GronthosS ShiMesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicineJ Dent Res88792806200919767575
2.	A GeorgeB SabsayPA SimonianA VeisCharacterization of a novel dentin matrix acidic phosphoprotein. Implications for induction of biomineralizationJ Biol Chem268126241263019938509401
3.	KL HirstK Ibaraki-O’ConnorMF YoungMJ DixonCloning and expression analysis of the bovine dentin matrix acidic phosphoprotein geneJ Dent Res76754760199710.1177/002203459707600307019109824
4.	M MacDougallTT GuX LuanD SimmonsJ ChenIdentification of a novel isoform of mouse dentin matrix protein 1: spatial expression in mineralized tissuesJ Bone Miner Res13422431199810.1359/jbmr.1998.13.3.4229525343
5.	C QinJC BrunnRG CookRS OrkiszewskiJP MaloneA VeisWT ButlerEvidence for the proteolytic processing of dentin matrix protein 1. Identification and characterization of processed fragments and cleavage sitesJ Biol Chem2783470034708200310.1074/jbc.M30531520012813042
6.	A AlmushaytK NarayananAE ZakiA GeorgeDentin matrix protein 1 induces cytodifferentiation of dental pulp stem cells into odontoblastsGene Ther13611620200610.1038/sj.gt.330268716319946
7.	M MacDougallBR DuPontD SimmonsRJ LeachAssignment of DMP1 to human chromosome 4 band q21 by in situ hybridizationCytogenet Cell Genet74189199610.1159/0001344108941370
8.	A GeorgeJ GuiNA JenkinsDJ GilbertNG CopelandA VeisIn situ localization and chromosomal mapping of the AG1 (Dmp1) geneJ Histochem Cytochem4215271531199410.1177/42.12.79833537983353
9.	HM AplinKL HirstAH CrosbyMJ DixonMapping of the human dentin matrix acidic phosphoprotein gene (DMP1) to the dentinogenesis imperfecta type II critical region at chromosome 4q21Genomics30347349199510.1006/geno.1995.98678586437
10.	G HeT DahlA VeisA GeorgeDentin matrix protein 1 initiates hydroxyapatite formation in vitroConnect Tissue Res44Suppl 1240245200310.1080/71371363712952204
11.	G HeS GajjeramanD SchultzD CooksonC QinWT ButlerJ HaoA GeorgeSpatially and temporally controlled biomineralization is facilitated by interaction between self-assembled dentin matrix protein 1 and calcium phosphate nuclei in solutionBiochemistry441614016148200510.1021/bi051045l
12.	K NarayananA RamachandranJ HaoG HeKW ParkM ChoA GeorgeDual functional roles of dentin matrix protein 1. Implications in biomineralization and gene transcription by activation of intracellular Ca²⁺ storeJ Biol Chem2781750017508200310.1074/jbc.M21270020012615915
13.	LW FisherNS FedarkoSix genes expressed in bones and teeth encode the current members of the SIBLING family of proteinsConnect Tissue Res44Suppl 1S33S40200310.1080/71371364412952171
14.	RN D’SouzaA CavenderG SunavalaJ AlvarezT OhshimaAB KulkarniM MacDougallGene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivoJ Bone Miner Res122040204919979421236
15.	KL HirstK Ibaraki-O’ConnorMF YoungMJ DixonCloning and expression analysis of the bovine dentin matrix acidic phosphoprotein geneJ Dent Res76754760199710.1177/002203459707600307019109824
16.	LP LimNC LauEG WeinsteinA AbdelhakimS YektaMW RhoadesCB BurgeDP BartelThe microRNAs of Caenorhabditis elegansGenes Dev1799110082003
17.	S RoushFJ SlackThe let-7 family of microRNAsTrends Cell Biol18505516200810.1016/j.tcb.2008.07.007
18.	XY HeJX ChenZ ZhangCL LiQL PengHM PengThe let-7a microRNA protects from growth of lung carcinoma by suppression of k-Ras and c-Myc in nude miceJ Cancer Res Clin Oncol13610231028201010.1007/s00432-009-0747-520033209
19.	S GronthosJ BrahimW LiLW FisherN ChermanA BoydeP DenBestenPG RobeyS ShiStem cell properties of human dental pulp stem cellsJ Dent Res81531535200210.1177/15440591020810080612147742
20.	BP LewisIH ShihMW Jones-RhoadesDP BartelCB BurgePrediction of mammalian microRNA targetsCell115787798200310.1016/S0092-8674(03)01018-314697198
21.	RM MarinJ VanicekEfficient use of accessibility in microRNA target predictionNucleic Acids Res391929201110.1093/nar/gkq76820805242
22.	SF MaddenSB CarpenterIB JefferyH BjorkbackaKA FitzgeraldLA O’NeillDG HigginsDetecting microRNA activity from gene expression dataBMC Bioinformatics11257201010.1186/1471-2105-11-25720482775
23.	ML CoubleJC FargesF BleicherB Perrat-MabillonM BoudeulleH MagloireOdontoblast differentiation of human dental pulp cells in explant culturesCalcif Tissue Int66129138200010.1007/PL0000583310652961
24.	LA GoffS BoucherCL RicuperoS FenstermacherM SwerdelLG ChaseCC AdamsJ ChesnutU LakshmipathyRP HartDifferentiating human multipotent mesenchymal stromal cells regulate microRNAs: prediction of microRNA regulation by PDGF during osteogenesisExp Hematol3613541369200810.1016/j.exphem.2008.05.004
25.	AL AbbottUncovering new functions for microRNAs in Caenorhabditis elegansCurr Biol21R668R671201110.1016/j.cub.2011.07.02721920301
26.	SM JohnsonSY LinFJ SlackThe time of appearance of the C. elegans let-7 microRNA is transcriptionally controlled utilizing a temporal regulatory element in its promoterDev Biol259364379200312871707
27.	AL AbbottE Alvarez-SaavedraEA MiskaNC LauDP BartelHR HorvitzV AmbrosThe let-7 MicroRNA family members mir-48, mir-84, and mir-241 function together to regulate developmental timing in Caenorhabditis elegansDev Cell9403414200510.1016/j.devcel.2005.07.00916139228
28.	H JinS LvJ YangX WangH HuC SuC ZhouJ LiY HuangL LiX LiuM WuQ QianUse of microRNA Let-7 to control the replication specificity of oncolytic adenovirus in hepatocellular carcinoma cellsPLoS One6e21307201110.1371/journal.pone.002130721814544
29.	Y LiTN VandenBoomD KongZ WangS AliPA PhilipFH SarkarUp-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cellsCancer Res6967046712200910.1158/0008-5472.CAN-09-129819654291
30.	AX ChenKD YuL FanJY LiC YangAJ HuangZM ShaoGermline genetic variants disturbing the Let-7/LIN28 double-negative feedback loop alter breast cancer susceptibilityPLoS Genet7e1002259201110.1371/journal.pgen.100225921912531
31.	J YunCA FrankenbergerWL KuoMC BoelensEM EvesN ChengH LiangWH LiH IshwaranAJ MinnMR RosnerSignalling pathway for RKIP and Let-7 regulates and predicts metastatic breast cancerEMBO J3045004541201110.1038/emboj.2011.31221873975
32.	L BoominathanThe guardians of the genome (p53, TA-p73, and TA-p63) are regulators of tumor suppressor miRNAs networkCancer Metastasis Rev29613639201010.1007/s10555-010-9257-920922462
33.	YS LeeA DuttaThe tumor suppressor microRNA let-7 represses the HMGA2 oncogeneGenes Dev2110251030200710.1101/gad.154040717437991
34.	SM JohnsonH GrosshansJ ShingaraM ByromR JarvisA ChengE LabourierKL ReinertD BrownFJ SlackRAS is regulated by the let-7 microRNA familyCell120635647200510.1016/j.cell.2005.01.01415766527
35.	S WangY TangH CuiX ZhaoX LuoW PanX HuangN ShenLet-7/miR-98 regulate Fas and Fas-mediated apoptosisGenes Immun12149154201110.1038/gene.2010.5321228813
36.	FE NicolasExperimental validation of microRNA targets using a luciferase reporter systemMethods Mol Biol732139152201110.1007/978-1-61779-083-6_1121431711
37.	X HuangS XuJ GaoF LiuJ YueT ChenB WumiRNA expression profiling identifies DSPP regulators in cultured dental pulp cellsInt J Mol Med28659667201121687927