Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii

  • Authors:
    • Binbin Hou
    • Zhenying Zhang
    • Fangliang Zheng
    • Xiaoming Liu
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  • Published online on: November 22, 2012     https://doi.org/10.3892/ijmm.2012.1193
  • Pages: 99-104
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Abstract

The dimorphism of Sporothrix schenckii (S. schenckii) reflects a developmental switch in morphology and lifestyle that is necessary for virulence. DRK1, a hybrid histidine kinase, functions as a global regulator of dimorphism and virulence in Blastomyces dermatitidis (B. dermatitidis) and Histoplasma capsulatum (H. capsulatum). The partial cDNA sequence of DRK1 of S. schenckii, designated SsDRK1, was obtained using degenerate primers based on the conserved domain of the DRK1 of other fungi. The complete cDNA sequence of SsDRK1 was obtained by 5' and 3' RACE. The full-length cDNA is 4743 bp in size and has an open reading frame (ORF) of 4071 bp, encoding 1356 amino acid residues. The predicted molecular mass of SsDRK1 is 147.3 kDa with an estimated theoretical isoelectric point of 5.46. The deduced amino acid sequence of SsDRK1 shows 65% identity to that of B. dermatitidis. The SsDRK1 was predicted to be a soluble histidine kinase and to contain three parts: sensor domain, linker domain and functional domain. Quantitative real-time RT-PCR revealed that SsDRK1 was more highly expressed in the yeast stage compared with that in the mycelial stage, which indicated that the SsDRK1 may be involved in the dimorphic switch in S. schenckii.
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January 2013
Volume 31 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Hou B, Zhang Z, Zheng F and Liu X: Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii . Int J Mol Med 31: 99-104, 2013.
APA
Hou, B., Zhang, Z., Zheng, F., & Liu, X. (2013). Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii . International Journal of Molecular Medicine, 31, 99-104. https://doi.org/10.3892/ijmm.2012.1193
MLA
Hou, B., Zhang, Z., Zheng, F., Liu, X."Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii ". International Journal of Molecular Medicine 31.1 (2013): 99-104.
Chicago
Hou, B., Zhang, Z., Zheng, F., Liu, X."Molecular cloning, characterization and differential expression of DRK1 in Sporothrix schenckii ". International Journal of Molecular Medicine 31, no. 1 (2013): 99-104. https://doi.org/10.3892/ijmm.2012.1193