Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles

Chen,Xuesong; Gu,Qi; Wang,Xiang; Ma,Qingwen; Tang,Huixiang; Yan,Xiaoshuang; Guo,Xinbing; Yan,Hao; Hao,Jie; Zeng,Fanyi

doi:10.3892/ijmm.2013.1372

Directed neuronal differentiation of mouse embryonic and induced pluripotent stem cells and their gene expression profiles

Authors:
- Xuesong Chen
- Qi Gu
- Xiang Wang
- Qingwen Ma
- Huixiang Tang
- Xiaoshuang Yan
- Xinbing Guo
- Hao Yan
- Jie Hao
- Fanyi Zeng
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Affiliations: Laboratory of Developmental Biology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China, College of Life Science, Northeast Agricultural University of China, Harbin 150030, P.R. China, Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University, Shanghai 200040, P.R. China, Shanghai Key Laboratory of Embryo and Reproduction Engineering and Key Laboratory of Embryo Molecular Biology, Ministry of Health, Shanghai 200040, P.R. China, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China
Published online on: May 8, 2013 https://doi.org/10.3892/ijmm.2013.1372
Pages: 25-34

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Abstract

Embryonic stem cells (ESCs) may be useful as a therapeutic source of cells for the production of healthy tissue; however, they are associated with certain challenges including immunorejection as well as ethical issues. Induced pluripotent stem cells (iPSCs) are a promising substitute since a patient's own adult cells would serve as tissue precursors. Ethical concerns prevent a full evaluation of the developmental potency of human ESCs and iPSCs, therefore, mouse iPSC models are required for protocol development and safety assessments. We used a modified culturing protocol to differentiate pluripotent cells from a mouse iPS cell line and two mouse ES cell lines into neurons. Our results indicated that all three pluripotent stem cell lines underwent nearly the same differentiation process when induced to form neurons in vitro. Genomic expression microarray profiling and single-cell RT-qPCR were used to analyze the neural lineage differentiation process, and more than one thousand differentially expressed genes involved in multiple molecular processes relevant to neural development were identified.

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