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Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot

  • Authors:
    • Wei Sheng
    • Yanyan Qian
    • Huijun Wang
    • Xiaojing Ma
    • Ping Zhang
    • Long Chen
    • Duan Ma
    • Guoying Huang

  • View Affiliations

  • Published online on: July 2, 2013     https://doi.org/10.3892/ijmm.2013.1427
  • Pages: 694-702
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Abstract

DNA methylation is catalyzed and maintained by DNA methyltransferases (DNMTs: DNMT1, DNMT3A and DNMT3B) and methyl-CpG-binding domain protein 2 (MBD2). However, little is known about the biological and clinical significance of the expression changes of DNMTs and MBD2 and their association with the methylation levels of long interspersed nuclear element-1 (LINE-1) in patients with tetralogy of Fallot (TOF). In this study, quantitative RT-PCR (qRT-PCR) was applied to analyze the mRNA levels of DNMTs and MBD2. The methylation status of LINE-1 was measured using the sequenom MassARRAY platform. The mRNA levels of the DNMTs and MBD2 showed a statistically significant decrease in the patients with TOF (P<0.001). The results also showed that patients with TOF had significantly lower global DNA methylation levels with a median of 61.50% [interquartile range (IQR), 59.78-63.77] compared with 63.54% (IQR, 62.49‑64.88) among the controls (P=0.0099). In the controls, only DNMT1 showed a significant positive correlation with the DNMT3A mRNA levels (r=0.718, P=0.002). Of note, the DNMT1, DNMT3A, DNMT3B and MBD2 mRNA levels positively correlated with each other; this was statistically significant (P<0.05). A significant positive correlation with the global DNA methylation status was observed only for MBD2 (r=-0.579, P=0.005) in patients with TOF. In conclusion, lower LINE-1 methylation levels significantly correlate with aberrant MBD2 mRNA levels. The lower expression of DNMT1 and DNMT3B may play an important role in the pathogenesis of TOF.
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September 2013
Volume 32 Issue 3

Print ISSN: 1107-3756
Online ISSN:1791-244X

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  • Impact Factor: 5.4
  • Ranked Medicine Research and Experimental
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  • CiteScore: 10.7
  • CiteScore Rank: Q1: #35/328 Biochemistry, Genetics and Molecular Biology
  • Source Normalized Impact per Paper (SNIP): 0.994
  • SCImago Journal Rank (SJR): 0.922
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Spandidos Publications style
Sheng W, Qian Y, Wang H, Ma X, Zhang P, Chen L, Ma D and Huang G: Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. Int J Mol Med 32: 694-702, 2013
APA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L. ... Huang, G. (2013). Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot. International Journal of Molecular Medicine, 32, 694-702. https://doi.org/10.3892/ijmm.2013.1427
MLA
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32.3 (2013): 694-702.
Chicago
Sheng, W., Qian, Y., Wang, H., Ma, X., Zhang, P., Chen, L., Ma, D., Huang, G."Association between mRNA levels of DNMT1, DNMT3A, DNMT3B, MBD2 and LINE-1 methylation status in infants with tetralogy of Fallot". International Journal of Molecular Medicine 32, no. 3 (2013): 694-702. https://doi.org/10.3892/ijmm.2013.1427