Long-term administration of ginsenoside Rh1 enhances learning and memory by promoting cell survival in the mouse hippocampus

Hou,Jingang; Xue,Jianjie; Lee,Mira; Yu,Jiaojiao; Sung,Changkeun

doi:10.3892/ijmm.2013.1552

Long-term administration of ginsenoside Rh1 enhances learning and memory by promoting cell survival in the mouse hippocampus

Authors:
- Jingang Hou
- Jianjie Xue
- Mira Lee
- Jiaojiao Yu
- Changkeun Sung
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Affiliations: Department of Food Science and Technology, College of Agriculture and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea
Published online on: November 8, 2013 https://doi.org/10.3892/ijmm.2013.1552
Pages: 234-240

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Abstract

Ginsenosides, the secondary plant metabolites produced by Panax ginseng are responsible for the enhancing effects on learning observed following treatment with Panax ginseng. A number of studies have provided correlational evidence that cell proliferation and survival are closely associated with hippocampal-dependent learning tasks. In this study, to investigate the beneficial effects of ginsenoside Rh1 on hippocampal cells and learning, mice (6 months old) were administered ginsenoside Rh1 at a dose of 5 and 10 mg/kg/day for a period of 3 months. Saline-treated mice were used as controls. The enhancement of memory and learning in the mice was evaluated by hippocampal-dependent tasks (passive avoidance tests and Morris water maze tests) and the immunohistochemical marker of cell proliferation, bromodeoxyuridine (BrdU). In addition, the levels of brain-derived neurotrophic factor (BDNF) were measured following treatment. Based on our data, the Rh1-treated group (5 and 10 mg/kg) showed a significantly improved learning and memory ability in the passive avoidance tests compared with the control group; however, only treatment with 10 mg/kg ginsenoside Rh1 significantly promoted spatial learning ability in the Morris water maze test. Ginsenoside Rh1 significantly enhanced cell survival in the dentate gyrus of mice, although it did not enhance hippocampal cell proliferation. In addition, ginsenoside Rh1 upregulated the expression of BDNF. These findings address the potential therapeutic significance of ginsenoside Rh1 as a nutritional supplement in memory loss and neurodegenerative diseases.

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