The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells

Ye,Zi; Wu,Wei; He,Min; Leong,Mancheong; Hu,Renming; Li,Yiming; Zhang,Shuo

doi:10.3892/ijmm.2014.1697

The effect of zipper‑interacting protein kinase on high glucose‑stimulated human aortic smooth muscle cells

Authors:
- Zi Ye
- Wei Wu
- Min He
- Mancheong Leong
- Renming Hu
- Yiming Li
- Shuo Zhang
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Affiliations: Institute of Endocrinology and Diabetology, Huashan Hospital, School of Medicine, Fudan University, Shanghai 200040, P.R. China, Department of Endocrinology, City University of Macau, Macau 000853, P.R. China
Published online on: March 14, 2014 https://doi.org/10.3892/ijmm.2014.1697
Pages: 1305-1311

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Abstract

Biologic abnormalities in vascular smooth muscle cells (VSMC) may perform a crucial role in the pathogenesis of diabetic vascular disease. The principal aim of this study was to determine the effects of zipper‑interacting protein kinase (ZIPK) on human aortic smooth muscle cells (HASMCs) stimulated by high glucose (HG). To elucidate the role of ZIPK in HG‑treated HASMCs, we overexpressed ZIPK by lentivirus infection and knocked down ZIPK by gene deletion using ZIPK shRNA. Flow cytometry and Cell Counting kit‑8 (CCK‑8) were separately used to analyze cell apoptosis and proliferation. Migratory activity was examined using transwell migration chamber assays. The results showed that ZIPK overexpression inhibited cell growth and migration, enhanced cell apoptosis, and reversed cell cycle disturbance by regulating the related proteins of cellular physiological process, such as human cell division cycle 14A phosphatase (Hcdc14A) and intercellular adhesion molecule 1 (ICAM‑1). In conclusion, the results suggested that ZIPK plays a role in HG‑treated HASMCs, indicating ZIPK is a potential therapeutic target for the treatment of diabetic vascular complications.

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1	Ruderman NB and Haudenschild C: Diabetes as an atherogenic factor. Prog Cardiovasc Dis. 26:373–412. 1984. View Article : Google Scholar : PubMed/NCBI
2	Srivastava AK: High glucose-induced activation of protein kinase signaling pathways in vascular smooth muscle cells: a potential role in the pathogenesis of vascular dysfunction in diabetes (review). Int J Mol Med. 9:85–89. 2002.
3	Blindt R, Krott N, Hanrath P, vom Dahl J, van Eys G and Bosserhoff AK: Expression patterns of integrins on quiescent and invasive smooth muscle cells and impact on cell locomotion. J Mol Cell Cardiol. 34:1633–1644. 2002. View Article : Google Scholar : PubMed/NCBI
4	Ross R: Cell biology of atherosclerosis. Annu Rev Physiol. 57:791–804. 1995. View Article : Google Scholar
5	Owens GK, Kumar MS and Wamhoff BR: Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol Rev. 84:767–801. 2004. View Article : Google Scholar : PubMed/NCBI
6	Kawai T, Matsumoto M, Takeda K, Sanjo H and Akira S: ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol. 18:1642–1651. 1998.PubMed/NCBI
7	Kögel D, Plöttner O, Landsberg G, Christian S and Scheidtmann KH: Cloning and characterization of Dlk, a novel serine/threonine kinase that is tightly associated with chromatin and phosphorylates core histones. Oncogene. 17:2645–2654. 1998.PubMed/NCBI
8	Brognard J, Zhang YW, Puto LA and Hunter T: Cancer-associated loss-of-function mutations implicate DAPK3 as a tumor-suppressing kinase. Cancer Res. 71:3152–3161. 2011. View Article : Google Scholar : PubMed/NCBI
9	Mills JC, Stone NL, Erhardt J and Pittman RN: Apoptotic membrane blebbing is regulated by myosin light chain phosphorylation. J Cell Biol. 140:627–636. 1998. View Article : Google Scholar : PubMed/NCBI
10	Coleman ML, Sahai EA, Yeo M, Bosch M, Dewar A and Olson MF: Membrane blebbing during apoptosis results from caspase-mediated activation of ROCK I. Nat Cell Biol. 3:339–345. 2001. View Article : Google Scholar : PubMed/NCBI
11	Engemann H, Heinzel V, Page G, Preuss U and Scheidtmann KH: DAP-like kinase interacts with the rat homolog of Schizosaccharomyces pombe CDC5 protein, a factor involved in pre-mRNA splicing and required for G2/M phase transition. Nucleic Acids Res. 30:1408–1417. 2002. View Article : Google Scholar
12	Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A and Hosoya H: ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. FEBS Lett. 451:81–84. 1999. View Article : Google Scholar : PubMed/NCBI
13	Niiro N and Ikebe M: Zipper-interacting protein kinase induces Ca(2+)-free smooth muscle contraction via myosin light chain phosphorylation. J Biol Chem. 276:29567–29574. 2001.PubMed/NCBI
14	Sato N, Kawai T, Sugiyama K, Muromoto R, Imoto S, Sekine Y, Ishida M, Akira S and Matsuda T: Physical and functional interactions between STAT3 and ZIP kinase. Int Immunol. 17:1543–1552. 2005. View Article : Google Scholar : PubMed/NCBI
15	Kakudo N, Shimotsuma A, Miyake S, Kushida S and Kusumoto K: Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. J Biomed Mater Res A. 84:191–197. 2008. View Article : Google Scholar : PubMed/NCBI
16	Kakudo N, Minakata T, Mitsui T, Kushida S, Notodihardjo FZ and Kusumoto K: Proliferation-promoting effect of platelet-rich plasma on human adipose-derived stem cells and human dermal fibroblasts. Plast Reconstr Surg. 122:1352–1360. 2008. View Article : Google Scholar : PubMed/NCBI
17	Yasunari K, Kohno M, Kano H, Yokokawa K, Minami M and Yoshikawa J: Mechanisms of action of troglitazone in the prevention of high glucoseinduced migration and proliferation of cultured coronary smooth muscle cells. Circ Res. 81:953–962. 1997. View Article : Google Scholar : PubMed/NCBI
18	Suh SJ, Jin UH, Kim SH, Chang HW, Son JK, Lee SH, Son KH and Kim CH: Ochnaflavone inhibits TNF-alpha-induced human VSMC proliferation via regulation of cell cycle, ERK1/2, and MMP-9. J Cell Biochem. 99:1298–1307. 2006. View Article : Google Scholar : PubMed/NCBI
19	Komatsu S and Ikebe M: ZIP kinase is responsible for the phosphorylation of myosin II and necessary for cell motility in mammalian fibroblasts. J Cell Biol. 165:243–254. 2004. View Article : Google Scholar : PubMed/NCBI
20	Cho YE, Ahn DS, Morgan KG and Lee YH: Enhanced contractility and myosin phosphorylation induced by Ca(2+)-independent MLCK activity in hypertensive rats. Cardiovasc Res. 91:162–170. 2011.
21	Usui T, Okada M, Hara Y and Yamawaki H: Death-associated protein kinase 3 mediates vascular inflammation and development of hypertension in spontaneously hypertensive rats. Hypertension. 60:1031–1039. 2012. View Article : Google Scholar : PubMed/NCBI
22	Piconi L, Quagliaro L, Da Ros R, Assaloni R, Giugliano D, Esposito K, Szabó C and Ceriello A: Intermittent high glucose enhances ICAM-1, VCAM-1, E-selectin and interleukin-6 expression in human umbilical endothelial cells in culture: the role of poly(ADP-ribose) polymerase. J Thromb Haemost. 2:1453–1459. 2004. View Article : Google Scholar
23	Martinelli R, Gegg M, Longbottom R, Adamson P, Turowski P and Greenwood J: ICAM-1-mediated endothelial nitric oxide synthase activation via calcium and AMP-activated protein kinase is required for transendothelial lymphocyte migration. Mol Biol Cell. 20:995–1005. 2009. View Article : Google Scholar
24	Vogt F, Zernecke A, Beckner M, Krott N, Bosserhoff AK, Hoffmann R, Zandvoort MA, Jahnke T, Kelm M, Weber C and Blindt R: Blockade of angio-associated migratory cell protein inhibits smooth muscle cell migration and neointima formation in accelerated atherosclerosis. J Am Coll Cardiol. 52:302–311. 2008. View Article : Google Scholar : PubMed/NCBI
25	Mailand N, Lukas C, Kaiser BK, Jackson PK, Bartek J and Lukas J: Deregulated human Cdc14A phosphatase disrupts centrosome separation and chromosome segregation. Nat Cell Biol. 4:317–322. 2002. View Article : Google Scholar
26	Kaiser BK, Zimmerman ZA, Charbonneau H and Jackson PK: Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdc14A phosphatase. Mol Biol Cell. 13:2289–2300. 2002. View Article : Google Scholar
27	Galli A, Svegliati-Baroni G, Ceni E, Milani S, Ridolfi F, Salzano R, Tarocchi M, Grappone C, Pellegrini G, Benedetti A, Surrenti C and Casini A: Oxidative stress stimulates proliferation and invasiveness of hepatic stellate cells via a MMP2-mediated mechanism. Hepatology. 41:1074–1084. 2005. View Article : Google Scholar : PubMed/NCBI
28	Shah PK: Role of inflammation and metalloproteinases in plaque disruption and thrombosis. Vasc Med. 3:199–206. 1998. View Article : Google Scholar : PubMed/NCBI
29	Tartakover-Matalon S, Cherepnin N, Kuchuk M, Drucker L, Kenis I, Fishman A, Pomeranz M and Lishner M: Impaired migration of trophoblast cells caused by simvastatin is associated with decreased membrane IGF-I receptor, MMP2 activity and HSP27 expression. Hum Reprod. 22:1161–1167. 2007. View Article : Google Scholar
30	Kaiser BK, Zimmerman ZA, Charbonneau H and Jackson PK: Disruption of centrosome structure, chromosome segregation, and cytokinesis by misexpression of human Cdc14A phosphatase. Mol Biol Cell. 13:2289–2300. 2002. View Article : Google Scholar : PubMed/NCBI