Identification of peptides that bind hepatitis C virus envelope protein E2 and inhibit viral cellular entry from a phage-display peptide library

  • Authors:
    • Xin Lü
    • Min Yao
    • Jian-Min Zhang
    • Jing Yang
    • Ying-Feng Lei
    • Xiao-Jun Huang
    • Zhan-Sheng Jia
    • Li Ma
    • Hai-Yun Lan
    • Zhi-Kai Xu
    • Wen Yin
  • View Affiliations

  • Published online on: February 25, 2014     https://doi.org/10.3892/ijmm.2014.1670
  • Pages: 1312-1318
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Abstract

Hepatitis C virus (HCV) envelope protein E2 is required for the entry of HCV into cells. Viral envelope proteins interact with cell receptors in a multistep process, which may be a promising target for the development of novel antiviral agents. In this study, a heptapeptide M13 phage-display library was screened for peptides that bind specifically to prokaryotically expressed, purified truncated HCV envelope protein E2. ELISA assay was used to quantify the binding of the peptides to HCV E2 protein. Flow cytometry, quantitative reverse-transcription PCR and western blotting were used to investigate the inhibition effect of one peptide on HCV infection in hepatoma cells (Huh7.5) in vitro. Four peptides capable of binding specifically to HCV E2 protein were obtained after three rounds of biopanning. Peptide C18 (WPWHNHR), with the highest affinity for binding HCV E2 protein, was synthesized. The results showed that peptide C18 inhibited the viral infectivity of both HCV pseudotype particles (HCVpp) harboring HCV envelope glycoproteins and cell-culture produced HCV (HCVcc). Thus, this study demonstrated that peptide C18 is a potential candidate for anti-HCV therapy as a novel viral entry inhibitor.
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May-2014
Volume 33 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Lü X, Yao M, Zhang J, Yang J, Lei Y, Huang X, Jia Z, Ma L, Lan H, Xu Z, Xu Z, et al: Identification of peptides that bind hepatitis C virus envelope protein E2 and inhibit viral cellular entry from a phage-display peptide library. Int J Mol Med 33: 1312-1318, 2014
APA
Lü, X., Yao, M., Zhang, J., Yang, J., Lei, Y., Huang, X. ... Yin, W. (2014). Identification of peptides that bind hepatitis C virus envelope protein E2 and inhibit viral cellular entry from a phage-display peptide library. International Journal of Molecular Medicine, 33, 1312-1318. https://doi.org/10.3892/ijmm.2014.1670
MLA
Lü, X., Yao, M., Zhang, J., Yang, J., Lei, Y., Huang, X., Jia, Z., Ma, L., Lan, H., Xu, Z., Yin, W."Identification of peptides that bind hepatitis C virus envelope protein E2 and inhibit viral cellular entry from a phage-display peptide library". International Journal of Molecular Medicine 33.5 (2014): 1312-1318.
Chicago
Lü, X., Yao, M., Zhang, J., Yang, J., Lei, Y., Huang, X., Jia, Z., Ma, L., Lan, H., Xu, Z., Yin, W."Identification of peptides that bind hepatitis C virus envelope protein E2 and inhibit viral cellular entry from a phage-display peptide library". International Journal of Molecular Medicine 33, no. 5 (2014): 1312-1318. https://doi.org/10.3892/ijmm.2014.1670