miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164

  • Authors:
    • Jia-Ang Shi
    • Da-Lin Lu
    • Xuan Huang
    • Wei Tan
  • View Affiliations

  • Published online on: April 22, 2014     https://doi.org/10.3892/ijmm.2014.1749
  • Pages: 237-243
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Abstract

It is known that microRNA-219 (miR-219) expression is downregulated in medulloblastoma. In the present study, we investigated the expression, targets and functional effects of miR-219 in D283-MED medulloblastoma cells. We first demonstrated that miR-219 not only inhibits proliferation, but also suppresses the invasion and migration of D283-MED cells. Moreover, the knockdown of miR-219 promoted the proliferation, migration and invasion of the D283-MED cells. Secondly, we predicted that miR-219 targets the 3' untranslated region (3'UTR) of CD164 and orthodenticle homeobox 2 (OTX2) and then confirmed that it significantly downregulated the protein expression of CD164 and OTX2 in D283-MED cells. Finally, we demonstrated that the proliferation, invasion and migration of D283-MED cells were promoted by theectopic expression of CD164. These results indicate that miR-219 suppresses the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. The results also suggest that miR-219 may serve as a potential therapeutic agent for medulloblastoma.
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July 2014
Volume 34 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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APA
Shi, J., Lu, D., Huang, X., & Tan, W. (2014). miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164. International Journal of Molecular Medicine, 34, 237-243. https://doi.org/10.3892/ijmm.2014.1749
MLA
Shi, J., Lu, D., Huang, X., Tan, W."miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164". International Journal of Molecular Medicine 34.1 (2014): 237-243.
Chicago
Shi, J., Lu, D., Huang, X., Tan, W."miR-219 inhibits the proliferation, migration and invasion of medulloblastoma cells by targeting CD164". International Journal of Molecular Medicine 34, no. 1 (2014): 237-243. https://doi.org/10.3892/ijmm.2014.1749