FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells

  • Authors:
    • Jun Soo Park
    • Wan Tae Kim
    • Shin Kim
    • Taeg Kyu Kwon
    • Byeong-Churl Jang
    • Eung-Seok Lee
    • Jong Wook Park
  • View Affiliations

  • Published online on: August 4, 2014     https://doi.org/10.3892/ijmm.2014.1875
  • Pages: 1051-1056
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Abstract

The inhibition of topoisomerase can suppress the growth of cancer cells and induce apoptosis. The aim of this study was to evaluate the anticancer effects and mechanisms of action of a novel topoisomerase inhibitor, 4-(furan-2-yl)-2-(pyridin-2-yl)-5,6-dihydro-1,10-phenanthroline (FPDHP). FPDHP suppressed the growth of Caki, A549, HT29 and MDA-MB-231 cells, and induced caspase-dependent apoptosis in the Caki cells. In particular, FPDHP also induced caspase-dependent apoptosis and the downregulation of the protein expression levels of cellular FLICE-like inhibitory protein (cFLIP) and the phosphorylation of Akt in Caki cells. Notably, the overexpression of cFLIP, but not that of Akt, in part, blocked the FPDHP-mediated apoptosis in Caki cells. In addition, FPDHP was further shown to induce the caspase-independent detachment of Caki cells from the culture dish; higher populations of apoptotic cells were observed in the detached cells than in the attached cells. To the best of our knoweledge, these results collectively demonstrate for the first time that FPDHP has a killing effect on Caki cells, which is mediated through both caspase-dependent apoptosis and caspase-independent cell detachment.
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October 2014
Volume 34 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Park JS, Kim WT, Kim S, Kwon TK, Jang B, Lee E and Park JW: FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells. Int J Mol Med 34: 1051-1056, 2014
APA
Park, J.S., Kim, W.T., Kim, S., Kwon, T.K., Jang, B., Lee, E., & Park, J.W. (2014). FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells. International Journal of Molecular Medicine, 34, 1051-1056. https://doi.org/10.3892/ijmm.2014.1875
MLA
Park, J. S., Kim, W. T., Kim, S., Kwon, T. K., Jang, B., Lee, E., Park, J. W."FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells". International Journal of Molecular Medicine 34.4 (2014): 1051-1056.
Chicago
Park, J. S., Kim, W. T., Kim, S., Kwon, T. K., Jang, B., Lee, E., Park, J. W."FPDHP, a novel anticancer agent, induces cell detachment and caspase-dependent apoptosis in Caki cells". International Journal of Molecular Medicine 34, no. 4 (2014): 1051-1056. https://doi.org/10.3892/ijmm.2014.1875