Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways

Kim,Sung  Ok; Park,Ji  Yeoung; Jeon,Seo  Young; Yang,Chea  Ha; Kim,Mi  Ryeo

doi:10.3892/ijmm.2015.2093

Saikosaponin a, an active compound of Radix Bupleuri, attenuates inflammation in hypertrophied 3T3-L1 adipocytes via ERK/NF-κB signaling pathways

Authors:
- Sung Ok Kim
- Ji Yeoung Park
- Seo Young Jeon
- Chea Ha Yang
- Mi Ryeo Kim
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Affiliations: Department of Herbal Pharmacology, College of Oriental Medicine, Daegu Haany University, Suseong‑gu, Daegu 706‑828, Republic of Korea, Department of Physiology, College of Oriental Medicine, Daegu Haany University, Suseong‑gu, Daegu 706‑828, Republic of Korea
Published online on: February 6, 2015 https://doi.org/10.3892/ijmm.2015.2093
Pages: 1126-1132

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Abstract

Bupleurum falcatum L. is employed in oriental medicine in Korea. This root has been used for anti‑inflammatory, anti‑pyretic, and anti‑hepatotoxic effects in the treatments of common cold, fever, and hepatitis. One of major bioactive compounds of Radix Bupleuri is the saikosaponin a (SSNa). However, little is known concerning the effects of SSNa on obesity associated with a state of low‑grade inflammation. Consequently, this study was conducted to determine the inhibition of the inflammation pathway of SSNa in obesity. MTT assay was conducted for cytotoxicity and viability; nuclear and cytoplasmic fractions were extracted from adipocytes for translocation of nuclear factor‑κB cells (NF‑κB); nitric oxide (NO) production and secretion using Griess reagent; reverse transcription‑polymerase chain reaction (RT‑PCR) and immunoblotting for mRNA and protein levels associated with inflammation in the hypertrophied adipocytes. The results revealed that SSNa significantly decreased the expression of tumor necrosis factor‑α (TNFα), interleukin (IL)‑1β and IL‑6 as proinflammatory cytokines, compared to that of non‑treated control cells. Inducible nitric oxide synthase (iNOS) and cyclooxygenase‑2 (COX‑2) as inflammatory factors were reduced by treatment of these cells with SSNa and also suppressed NO production. Phosphorylation of IκBα was inhibited and translocation of NF‑κB was suppressed via the ERK pathway in response to SSNa treatment. In conclusion, the results demonstrated that SSNa can inhibit the expression of inflammatory‑associatied genes in hypertrophied 3T3‑L1 adipocytes and is a potent inhibitor of NF‑κB activation. Thus these results suggest that SSNa is a novel therapeutic agent against that can be used against obesity‑associated inflammation.

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