Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands

  • Authors:
    • Hyunkeun Song
    • Yeonye Kim
    • Gabin Park
    • Yeong-Seok Kim
    • Seonghan Kim
    • Hyun-Kyung Lee
    • Woo Yeong Chung
    • Seok Ju Park
    • Sang-Youb Han
    • Daeho Cho
    • Daeyoung Hur
  • View Affiliations

  • Published online on: August 20, 2015     https://doi.org/10.3892/ijmm.2015.2317
  • Pages: 1180-1188
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Abstract

Transforming growth factor-β (TGF-β) has a significant role in the response to injury and tissue repair, and it has been detected in various cell types. However, the mechanism by which it regulates the response to ischemia‑reperfusion injury (IRI) and manipulates natural killer (NK) cells is not well understood. In the present study, TGF‑β modulated NK cell function, thereby promoting recovery from renal IRI. Human renal proximal tubular epithelial cells (HK‑2) treated with TGF‑β exhibited increased surface and intracellular expression of the NK group 2 member D (NKG2D) ligand MICA. This increased surface expression of MICA inhibited NK cell cytotoxicity to the HK‑2 cells. In addition, an enzyme‑linked immunosorbent assay revealed that TGF‑β treatment evidently increased the amount of soluble MICA released into the culture supernatant from HK‑2 cells. Taken together, these findings suggest that TGF‑β‑induced release of soluble MICA leads to downregulation of NKG2D, thereby preventing NK cell‑mediated cytotoxicity toward renal proximal tubular epithelial cells in renal IRI, which in turn improves the survival of these cells.
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October-2015
Volume 36 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Song H, Kim Y, Park G, Kim Y, Kim S, Lee H, Chung WY, Park SJ, Han S, Cho D, Cho D, et al: Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. Int J Mol Med 36: 1180-1188, 2015
APA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H. ... Hur, D. (2015). Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands. International Journal of Molecular Medicine, 36, 1180-1188. https://doi.org/10.3892/ijmm.2015.2317
MLA
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36.4 (2015): 1180-1188.
Chicago
Song, H., Kim, Y., Park, G., Kim, Y., Kim, S., Lee, H., Chung, W. Y., Park, S. J., Han, S., Cho, D., Hur, D."Transforming growth factor-β1 regulates human renal proximal tubular epithelial cell susceptibility to natural killer cells via modulation of the NKG2D ligands". International Journal of Molecular Medicine 36, no. 4 (2015): 1180-1188. https://doi.org/10.3892/ijmm.2015.2317