Tectonic‑1 contributes to the growth and migration of prostate cancer cells in vitro

Wang,Zhijun; Gao,Yi; Liu,Yushan; Chen,Jie; Wang,Junkai; Gan,Sishun; Xu,Danfeng; Cui,Xingang

doi:10.3892/ijmm.2015.2313

Tectonic‑1 contributes to the growth and migration of prostate cancer cells in vitro

Authors:
- Zhijun Wang
- Yi Gao
- Yushan Liu
- Jie Chen
- Junkai Wang
- Sishun Gan
- Danfeng Xu
- Xingang Cui
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Affiliations: Department of Urology, Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China
Published online on: August 14, 2015 https://doi.org/10.3892/ijmm.2015.2313
Pages: 931-938
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Tectonic‑1 (TCTN1) is an upstream gene involved in embryonic development. The aim of the present study was to investigate the effect of the TCTN1 gene on the viability and migration of prostate cancer cells. Lentivirus‑mediated short hairpin RNA (shRNA) was constructed to silence the expression of TCTN1 in PC‑3 and DU145 prostate cancer cells. Cell viability and proliferation were measured using MTT and colony formation assays, and the distribution of cells in phases of the cell cycle was determined using flow cytometry. Cell migration was detected using a Transwell assay. The results demonstrated that TCTN1 was widely expressed in several human prostate cancer cell lines. Knockdown of the TCTN1 gene by RNA interference markedly suppressed cell viability and colony formation in the PC‑3 and DU145 cell lines. Cell cycle progression was also arrested by TCTN1 silencing. In addition, knockdown of the TCTN1 gene led to the inhibition of cell migration in the two cell lines. These findings confirmed the direct association between the TCTN1 gene and prostate cancer growth in vitro. With further understanding and clinical investigation, this indicates the potential for future development of a novel marker for early detection and gene therapy for prostate cancer.

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