Open Access

Docosahexenoic acid treatment ameliorates cartilage degeneration via a p38 MAPK-dependent mechanism

  • Authors:
    • Zhenzhong Wang
    • Ai Guo
    • Lifeng Ma
    • Haomiao Yu
    • Liang Zhang
    • Hai Meng
    • Yinpeng Cui
    • Fei Yu
    • Bo Yang
  • View Affiliations

  • Published online on: April 14, 2016     https://doi.org/10.3892/ijmm.2016.2567
  • Pages: 1542-1550
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Osteoarthritis (OA) is a common chronic inflammatory disease, characterized by cartilage degradation. The aberrant expression of matrix metalloproteinase-13 (MMP-13) plays a vital role in the pathogenesis of OA. The anti‑inflammatory property of docosahexenoic acid (DHA) was previously revealed and showed that DHA retards the progress of many types of inflammatory disease. To evaluate the prophylactic function of DHA in OA, the effect of DHA on cartilage degeneration was assessed in interleukin‑1β (IL‑1β) stimulated human chondrosarcoma SW1353 cells or a rat model of adjuvant‑induced arthritis (AIA). The safe concentration range (0‑50 µg/ml in vitro) of DHA was determined by flow cytometry and MTT assay. The inhibitory effects of DHA on MMP‑13 mRNA and protein expression were confirmed by RT‑qPCR, ELISA and western blotting. Furthermore, findings of an in vivo study showed that DHA can increase the thickness of articular cartilage and decrease MMP‑13 expression in cartilage matrix in a rat AIA model. We also revealed the mechanism by which DHA ameliorates cartilage degeneration from OA. The DHA-mediated inhibition of MMP‑13 expression was partially attributed to the inactivation of the p38 mitogen‑activated protein kinases pathway by suppressing p‑p38 in IL-1β-stimulated SW1353 cells and a rat AIA model. Our findings suggested that DHA is a promising therapeutic agent that may be used for the prevention and treatment of OA.
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June-2016
Volume 37 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Wang Z, Guo A, Ma L, Yu H, Zhang L, Meng H, Cui Y, Yu F and Yang B: Docosahexenoic acid treatment ameliorates cartilage degeneration via a p38 MAPK-dependent mechanism. Int J Mol Med 37: 1542-1550, 2016
APA
Wang, Z., Guo, A., Ma, L., Yu, H., Zhang, L., Meng, H. ... Yang, B. (2016). Docosahexenoic acid treatment ameliorates cartilage degeneration via a p38 MAPK-dependent mechanism. International Journal of Molecular Medicine, 37, 1542-1550. https://doi.org/10.3892/ijmm.2016.2567
MLA
Wang, Z., Guo, A., Ma, L., Yu, H., Zhang, L., Meng, H., Cui, Y., Yu, F., Yang, B."Docosahexenoic acid treatment ameliorates cartilage degeneration via a p38 MAPK-dependent mechanism". International Journal of Molecular Medicine 37.6 (2016): 1542-1550.
Chicago
Wang, Z., Guo, A., Ma, L., Yu, H., Zhang, L., Meng, H., Cui, Y., Yu, F., Yang, B."Docosahexenoic acid treatment ameliorates cartilage degeneration via a p38 MAPK-dependent mechanism". International Journal of Molecular Medicine 37, no. 6 (2016): 1542-1550. https://doi.org/10.3892/ijmm.2016.2567