Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis

  • Authors:
    • Jin Liu
    • Xiangya Ding
    • Li Yuan
    • Xiaojun Zhang
  • View Affiliations

  • Published online on: June 14, 2016     https://doi.org/10.3892/ijmm.2016.2641
  • Pages: 529-536
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Abstract

Pterygium is a common degenerative and proliferative disease of the ocular surface. It becomes a significant sight-threatening complication once its ingrowth covers the pupil. The proliferative capacities of pterygial cells give pterygia the appearance of having a mechanism similar to tumorigenesis. Long non-coding RNAs (lncRNAs) are key regulators of gene expression. The expression levels of certain lncRNAs are associated with a number of diseases, such as different tumors and metabolic disorders, among others. However, the contributions of lncRNAs to pterygium remain largely unexplored. In this study, we constructed pterygium-related lncRNA libraries using microarray analysis to investigate the potential roles of lncRNAs in the development of pterygium. A total of 3,066 upregulated and 1,646 downregulated lncRNAs were identified in pterygium tissues compared with paired adjacent normal conjunctival tissues (log fold change >2.0). Quantitative polymerase chain reaction (qPCR) was performed to validate 3 upregulated and 2 downregulated lncRNAs in 10 patients. Bioinformatics analyses (Gene Ontology analysis and pathway analysis) were used for further research. Pathway analysis indicated that 82 pathways corresponded to the downregulated transcripts and that 15 pathways corresponded to the upregulated transcripts (p-value cut-off, 0.05). Our results reveal differentially expressed lncRNAs in pterygium and suggest that lncRNAs may be the novel molecular targets for the treatment of pterygium.
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August-2016
Volume 38 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Liu J, Ding X, Yuan L and Zhang X: Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis. Int J Mol Med 38: 529-536, 2016.
APA
Liu, J., Ding, X., Yuan, L., & Zhang, X. (2016). Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis. International Journal of Molecular Medicine, 38, 529-536. https://doi.org/10.3892/ijmm.2016.2641
MLA
Liu, J., Ding, X., Yuan, L., Zhang, X."Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis". International Journal of Molecular Medicine 38.2 (2016): 529-536.
Chicago
Liu, J., Ding, X., Yuan, L., Zhang, X."Identification of pterygium-related long non-coding RNAs and expression profiling by microarray analysis". International Journal of Molecular Medicine 38, no. 2 (2016): 529-536. https://doi.org/10.3892/ijmm.2016.2641