Open Access

Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling

  • Authors:
    • Xiao‑Long Lin
    • Mihua Liu
    • Yuanbo Liu
    • Huijun Hu
    • Yongquan  Pan
    • Weiwen Zou
    • Xiaojuan Fan
    • Xuemei Hu
  • View Affiliations

  • Published online on: November 1, 2017     https://doi.org/10.3892/ijmm.2017.3228
  • Pages:129-136
  • Copyright: © Lin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Transforming growth factor β1 (TGFβ1) is a cytokine with multiple functions. TGFβ1 significantly induces migration and invasion of liver cancer cells. However, the molecular mechanisms underlying this effect remain unclear. Epithelial‑to‑mesenchymal transition (EMT) is crucial for the development of invasion and metastasis in human cancers. The aim of the present study was to determine whether TGFβ1‑induced EMT promoted migration and invasion in HepG2 cells. The underlying mechanism and the effect of EMT on HepG2 cells were also investigated. The results demonstrated that TGFβ1 may induce EMT to promote migration and invasion of HepG2 cells, and this effect depends on activation of the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) signaling pathway. JAK/STAT3 signaling is involved in human malignancies, including lung cancer, and is implicated in cell transformation, tumorigenicity, EMT and metastasis. In the present study, TGFβ1 also activated JAK/STAT3 signaling in HepG2 cells and promoted Twist expression, but these events were abolished by treatment with the STAT3 inhibitor AG490. Additionally, Twist siRNA blocked TGFβ1‑induced EMT. Thus, TGFβ1 was shown to induce EMT, thereby promoting the migration and invasion of HepG2 cells via JAK/STAT3/Twist signaling.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W. ... Hu, X. (2018). Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling. International Journal of Molecular Medicine, 41, 129-136. https://doi.org/10.3892/ijmm.2017.3228
MLA
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41.1 (2018): 129-136.
Chicago
Lin, X., Liu, M., Liu, Y., Hu, H., Pan, Y., Zou, W., Fan, X., Hu, X."Transforming growth factor β1 promotes migration and invasion in HepG2 cells: Epithelial‑to‑mesenchymal transition via JAK/STAT3 signaling". International Journal of Molecular Medicine 41, no. 1 (2018): 129-136. https://doi.org/10.3892/ijmm.2017.3228