Open Access

Cryptdin-2 predicts intestinal injury during heatstroke in mice

  • Authors:
    • Jingjing Ji
    • Zhengtao Gu
    • Hui Li
    • Lei Su
    • Zhifeng Liu
  • View Affiliations

  • Published online on: November 1, 2017     https://doi.org/10.3892/ijmm.2017.3229
  • Pages:137-146
  • Copyright: © Ji et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Intestinal injury-induced bacterial translocation and endotoxemia are important in the pathophysiological process of heatstroke. However, the underlying mechanism remains to be fully elucidated. Previous studies using 2D-gel electrophoresis found that defensin-related cryptdin-2 (Cry-2), an intestinal α-defensin, is upregulated in intestinal tissues during heatstroke in mice, and that treatment with ulinastatin, a multivalent enzyme inhibitor, reduced heat-induced acute lung injury. To investigate the association between Cry-2 and heat stress (HS)-induced intestinal injury and the probable protective role of ulinastatin, the present study examined the intestinal expression of Cry-2 via histopathologic analysis and reverse transcription-quantitative polymerase chain reaction analysis in mice with heatstroke. The heat-stressed mice were exposed to different core temperatures and cooling treatments, and intestinal pathological changes and Chiu scores were determined. Chemical markers of intestinal injury, serum and intestinal concentrations of diamine oxidase (DAO) and D-lactic acid (D-Lac), and serum and intestinal concentrations of Cry-2 were also determined. Correlations were analyzed using Spearman's correlation analysis. It was found that HS upregulated the expression of Cry-2, and the serum and intestinal concentrations of Cry-2 were correlated with the severity of HS-induced intestinal damage, indicated by pathology scores and concentrations of DAO and D-lac. Ulinastatin protected the intestines from HS-induced injury and downregulated the expression of Cry-2, which was also correlated with the extent of intestinal injury. Therefore, ulinastatin administration may be beneficial for patients with heatstroke, and Cry-2 may be a novel predictor of HS-induced intestinal injury.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Ji, J., Gu, Z., Li, H., Su, L., & Liu, Z. (2018). Cryptdin-2 predicts intestinal injury during heatstroke in mice. International Journal of Molecular Medicine, 41, 137-146. https://doi.org/10.3892/ijmm.2017.3229
MLA
Ji, J., Gu, Z., Li, H., Su, L., Liu, Z."Cryptdin-2 predicts intestinal injury during heatstroke in mice". International Journal of Molecular Medicine 41.1 (2018): 137-146.
Chicago
Ji, J., Gu, Z., Li, H., Su, L., Liu, Z."Cryptdin-2 predicts intestinal injury during heatstroke in mice". International Journal of Molecular Medicine 41, no. 1 (2018): 137-146. https://doi.org/10.3892/ijmm.2017.3229