Open Access

Genome-wide DNA methylome alterations in acute coronary syndrome

  • Authors:
    • Dandan Li
    • Jing Yan
    • Yunlong Yuan
    • Cheng Wang
    • Jia Wu
    • Qingwen Chen
    • Jiaxi Song
    • Junjun Wang
  • View Affiliations

  • Published online on: October 27, 2017     https://doi.org/10.3892/ijmm.2017.3220
  • Pages:220-232
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Acute coronary syndrome (ACS) is a common disease with high mortality and morbidity rates. The methylation status of blood DNA may serve as a potential early diagnosis and prevention biomarker for numerous diseases. The present study was designed to explore novel genome-wide aberrant DNA methylation patterns associated with ACS. The Infinium HumanMethylation450 assay was used to examine genome-wide DNA methylation profiles in 3 pairs of ACS and control group samples. Epigenome-wide DNA methylation, genomic distribution, Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. The results were confirmed using methylation-specific polymerase chain reaction (MSP) and Sequenom MassARRAY analyses in ACS, stable coronary artery disease (SCAD) and control samples. A total of 11,342 differentially methylated (DM) 5'-C-phosphate-G-3' (CpG) sites were identified, including 8,865 hypomethylated and 2,477 hypermethylated CpG sites in the ACS group compared with the control samples. They varied in frequency across genomic compartments, but were particularly notable in gene bodies and shores. The results of GO term and KEGG pathway enrichment analyses revealed that the methylated genes were associated with certain biological processes and pathways. Despite the considerable variability in methylation data, the candidate selected possessed significant methylation alteration in mothers against decapentaplegic homolog 3 (SMAD3) transcription start site 155 (Chr1:67356838-Chr1:67356942). MSP analysis from 81 ACS samples, 74 SCAD samples and 53 healthy samples, and Sequenom MassARRAY analysis, confirmed that differential CpG methylation of SMAD3 was significantly corrected with the reference results of the HumanMethylation450 array. The data identified an ACS-specific DNA methylation profile with a large number of novel DM CpG sites, some of which may serve as candidate markers for the early diagnosis of ACS.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Li, D., Yan, J., Yuan, Y., Wang, C., Wu, J., Chen, Q. ... Wang, J. (2018). Genome-wide DNA methylome alterations in acute coronary syndrome. International Journal of Molecular Medicine, 41, 220-232. https://doi.org/10.3892/ijmm.2017.3220
MLA
Li, D., Yan, J., Yuan, Y., Wang, C., Wu, J., Chen, Q., Song, J., Wang, J."Genome-wide DNA methylome alterations in acute coronary syndrome". International Journal of Molecular Medicine 41.1 (2018): 220-232.
Chicago
Li, D., Yan, J., Yuan, Y., Wang, C., Wu, J., Chen, Q., Song, J., Wang, J."Genome-wide DNA methylome alterations in acute coronary syndrome". International Journal of Molecular Medicine 41, no. 1 (2018): 220-232. https://doi.org/10.3892/ijmm.2017.3220