Open Access

Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia

  • Authors:
    • Min Zhao
    • Shimiao Tang
    • Junchi Xin
    • Yingliang Wei
    • Dongyan Liu
  • View Affiliations

  • Published online on: November 9, 2017     https://doi.org/10.3892/ijmm.2017.3247
  • Pages:322-330
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: HTML 0 views | PDF 0 views
0

Abstract

Long-term therapeutic hyperoxia may exert serious toxic effects on intestinal epithelial cells in vitro and in vivo. The aim of the present study was to investigate the cause of this intestinal injury under conditions of hyperoxia. Caco-2 cells were treated with different concentrations of hydrogen peroxide (H2O2) and 85% hyperoxia for 24 h. higher rates of injury of Caco-2 cells were observed in the hyperoxia and H2O2 groups compared with the control group. The reactive oxygen species (ROS) level of the hyperoxia group was significantly higher compared with that of the 400 µM H2O2 group. The protein and gene levels of RelA, RelB, hypoxia‑inducible factor-1α, tumor necrosis factor-α and apoptosis signal‑regulating kinase 1 were significantly higher in the hyperoxia and H2O2 groups compared with those in the control group. In conclusion, during hyperoxia, intestinal epithelial cells were destroyed and the levels of ROS were increased. Therefore, ROS may play an important role in intestinal injury in a hyperoxic environment.

Related Articles

Journal Cover

January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Zhao, M., Tang, S., Xin, J., Wei, Y., & Liu, D. (2018). Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia. International Journal of Molecular Medicine, 41, 322-330. https://doi.org/10.3892/ijmm.2017.3247
MLA
Zhao, M., Tang, S., Xin, J., Wei, Y., Liu, D."Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia". International Journal of Molecular Medicine 41.1 (2018): 322-330.
Chicago
Zhao, M., Tang, S., Xin, J., Wei, Y., Liu, D."Reactive oxygen species induce injury of the intestinal epithelium during hyperoxia". International Journal of Molecular Medicine 41, no. 1 (2018): 322-330. https://doi.org/10.3892/ijmm.2017.3247