Open Access

Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells

  • Authors:
    • Xiao Yin
    • Zhaobin Zeng
    • Jinliang Xing
    • Ansheng Zhang
    • Wenkai Jiang
    • Wei Wang
    • Hantang Sun
    • Longxing Ni
  • View Affiliations

  • Published online on: November 13, 2017     https://doi.org/10.3892/ijmm.2017.3254
  • Pages:331-339
  • Copyright: © Yin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Substantial evidence has indicated that Notch and bone morphogenetic protein (BMP) signaling may regulate odontoblastic differentiation. Hairy/enhancer‑of‑split related with YRPW motif 1 (Hey1), a downstream target gene of Notch and BMP signaling, is expressed in dental pulp tissues and has been demonstrated to be responsible for osteoblast mineralization. The aim of this study was to investigate the effects of Hey1 on odontoblast differentiation. The results of the study demonstrated that Hey1 expression in odontoblast‑lineage cells (OLCs) was upregulated by stimulation of osteoblastic/odontoblastic differentiation medium containing ascorbic acid, β‑glycerol phosphate and dexamethasone. Furthermore, stable Hey1‑overexpressing cells expressed higher levels of dentin sialophosphoprotein (DSPP) and exhibited higher mineralization capabilities following stimulation by differentiation medium. Furthermore, RNA interference‑mediated knockdown of Hey1 downregulated the expression levels of DSPP in OLCs stimulated by differentiation medium. Taken together, the findings indicate that Hey1 may be a positive regulator of odontoblastic differentiation. The present study broadens the understanding of odontoblast differentiation and biomineralization.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Yin, X., Zeng, Z., Xing, J., Zhang, A., Jiang, W., Wang, W. ... Ni, L. (2018). Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells. International Journal of Molecular Medicine, 41, 331-339. https://doi.org/10.3892/ijmm.2017.3254
MLA
Yin, X., Zeng, Z., Xing, J., Zhang, A., Jiang, W., Wang, W., Sun, H., Ni, L."Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells". International Journal of Molecular Medicine 41.1 (2018): 331-339.
Chicago
Yin, X., Zeng, Z., Xing, J., Zhang, A., Jiang, W., Wang, W., Sun, H., Ni, L."Hey1 functions as a positive regulator of odontogenic differentiation in odontoblast‑lineage cells". International Journal of Molecular Medicine 41, no. 1 (2018): 331-339. https://doi.org/10.3892/ijmm.2017.3254