Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells

  • Authors:
    • Yourim Lim
    • Ji-Won Park
    • Ok-Kyoung Kwon
    • Jae-Won Lee
    • Han-Sol Lee
    • Sangwoo Lee
    • Sangho Choi
    • Wanyi Li
    • Hang Jin
    • Sang-Bae Han
    • Kyung-Seop Ahn
  • View Affiliations

  • Published online on: October 27, 2017     https://doi.org/10.3892/ijmm.2017.3223
  • Pages:391-398
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Abstract

Castanea extracts are known to have antioxidant properties and are used as a traditional medicine in China and Asia. However, the biological activity of Castanea seguinii Dode has remained to be fully elucidated. The present study investigated the anti-inflammatory effects of a Castanea seguinii Dode methanolic extract (CSME) on lipopolysaccharide-induced RAW264.7 macrophage cells. CSME inhibited the production of nitric oxide (NO) and the expression of inducible NO synthase. It also suppressed the production of the pro-inflammatory cytokines inteleukin-6 and tumor necrosis factor-α, as well as chemokine monocyte chemoattractant protein 1. In addition, CSME inhibited nuclear factor-κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling, while also downregulating transcription factor activator protein-1. Furthermore, CSME increased heme oxygenase 1 through the upregulation of NF (erythroid-derived 2)-like-2 (Nrf-2), which directly or indirectly affects inflammation. It also increased the phosphorylation of 5'-adenosine monophosphate-activated protein kinase (AMPK). In conclusion, CSME was demonstrated to exert its anti-inflammatory activities through the inhibition of the NF-κB and the MAPK signaling pathways, as well as the activation of Nrf-2 and AMPK. These results indicated that CSME may be a promising for development as a commercial anti-inflammatory medicine.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S. ... Ahn, K. (2018). Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells. International Journal of Molecular Medicine, 41, 391-398. https://doi.org/10.3892/ijmm.2017.3223
MLA
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41.1 (2018): 391-398.
Chicago
Lim, Y., Park, J., Kwon, O., Lee, J., Lee, H., Lee, S., Choi, S., Li, W., Jin, H., Han, S., Ahn, K."Anti-inflammatory effects of a methanolic extract of Castanea seguinii Dode in LPS-induced RAW264.7 macrophage cells". International Journal of Molecular Medicine 41, no. 1 (2018): 391-398. https://doi.org/10.3892/ijmm.2017.3223