Open Access

Proliferation of vascular smooth muscle cells under inflammation is regulated by NF-κB p65/microRNA-17/RB pathway activation

  • Authors:
    • Dong Yang
    • Chen Sun
    • Jing Zhang
    • Shu Lin
    • Lin Zhao
    • Lun Wang
    • Ruoran Lin
    • Junyuan Lv
    • Shijie Xin
  • View Affiliations

  • Published online on: October 25, 2017     https://doi.org/10.3892/ijmm.2017.3212
  • Pages:43-50
  • Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inflammation and excessive proliferation of vascular smooth muscle cells (VSMCs) have key roles in various vascular disorders, including restenosis, atherosclerosis and pulmonary artery hypertension. However, the underlying mechanism remains unclear. The present study investigated the role of nuclear factor-κB (NF-κB) and microRNA (miRNA) in the regulation of VSMC proliferation under inflammatory conditions. It was demonstrated that miR‑17 stimulated the proliferation of VSMCs, enhanced cell cycle G1/S transition, and increased levels of proliferating cell nuclear antigen and E2F1. By directly targeting the retinoblastoma (RB) protein mRNA-3' untranslated region, miR‑17 suppressed the expression of RB. Activation of NF-κB p65 resulted in increased miR‑17 expression in VSMCs, whereas inactivation of NF-κB p65 resulted in decreased expression of miR‑17 in VSMCs. NF-κB p65 signalling directly regulates miR‑17 promoter activity. NF-κB p65 activation also suppressed RB expression, which was abrogated by miR‑17 inhibitor. Taken together, the present results indicated that VSMC proliferation is regulated by activation of the NF-κB p65/miR‑17/RB pathway. As NF-κB p65 signalling is activated in and is a master regulator of the inflammatory response, the present findings may provide a mechanism for the excessive proliferation of VSMCs under inflammation during vascular disorders and may identify novel targets for the treatment of vascular diseases.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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Copy and paste a formatted citation
APA
Yang, D., Sun, C., Zhang, J., Lin, S., Zhao, L., Wang, L. ... Xin, S. (2018). Proliferation of vascular smooth muscle cells under inflammation is regulated by NF-κB p65/microRNA-17/RB pathway activation. International Journal of Molecular Medicine, 41, 43-50. https://doi.org/10.3892/ijmm.2017.3212
MLA
Yang, D., Sun, C., Zhang, J., Lin, S., Zhao, L., Wang, L., Lin, R., Lv, J., Xin, S."Proliferation of vascular smooth muscle cells under inflammation is regulated by NF-κB p65/microRNA-17/RB pathway activation". International Journal of Molecular Medicine 41.1 (2018): 43-50.
Chicago
Yang, D., Sun, C., Zhang, J., Lin, S., Zhao, L., Wang, L., Lin, R., Lv, J., Xin, S."Proliferation of vascular smooth muscle cells under inflammation is regulated by NF-κB p65/microRNA-17/RB pathway activation". International Journal of Molecular Medicine 41, no. 1 (2018): 43-50. https://doi.org/10.3892/ijmm.2017.3212