Anti-neuroinflammatory effect of curcumin on Pam3CSK4-stimulated microglial cells

  • Authors:
    • Meiling Jin
    • Sun Young Park
    • Qian Shen
    • Yihong Lai
    • Xingmei Ou
    • Zhuo Mao
    • Dongxu Lin
    • Yangyang Yu
    • Weizhen Zhang
  • View Affiliations

  • Published online on: October 27, 2017     https://doi.org/10.3892/ijmm.2017.3217
  • Pages:521-530
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Abstract

Curcumin is the main curcuminoid present in Curcuma longa and it has been previously reported to exhibit a wide range of pharmacological activities. In the present study, the inhibitory effects of curcumin on the inflammatory mediators released by Pam3CSK4-stimulated BV-2 microglial cells were investigated. The production of pro-inflammatory mediators and cytokines, including tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2), were measured by enzyme‑linked immunosorbent assay (ELISA). The expression of inflammatory genes, including inducible nitric oxide synthase and cyclooxygenase-2, were further investigated using reverse transcription-quantitative polymerase chain reaction. The effects of curcumin on heme oxygenase-1 (HO-1), nuclear factor (erythroid-derived 2)-like 2 (Nrf2), mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) signaling pathways were analyzed by western blotting. The results revealed that curcumin dose-dependently inhibited Pam3CSK4-induced nitric oxide, PGE2, and TNF-α secretion. Curcumin suppressed the secretion of inflammatory mediators through an increase in the expression of HO-1. Curcumin induced HO-1 transcription and translation through the Nrf2/antioxidant response element signaling pathway. Inhibitory experiments revealed that HO-1 was required for the anti-inflammatory effects of curcumin. Further mechanistic studies demonstrated that curcumin inhibited neuroinflammation by suppressing NF-κB and MAPK signaling pathways in Pam3CSK4-activated microglial cells. The results of the present study suggest that curcumin may be a novel treatment for neuroinflammation-mediated neurodegenerative disorders.

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January 2018
Volume 41 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2016 Impact Factor: 2.341
Ranked #21/128 Medicine Research and Experimental
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APA
Jin, M., Park, S.Y., Shen, Q., Lai, Y., Ou, X., Mao, Z. ... Zhang, W. (2018). Anti-neuroinflammatory effect of curcumin on Pam3CSK4-stimulated microglial cells. International Journal of Molecular Medicine, 41, 521-530. https://doi.org/10.3892/ijmm.2017.3217
MLA
Jin, M., Park, S. Y., Shen, Q., Lai, Y., Ou, X., Mao, Z., Lin, D., Yu, Y., Zhang, W."Anti-neuroinflammatory effect of curcumin on Pam3CSK4-stimulated microglial cells". International Journal of Molecular Medicine 41.1 (2018): 521-530.
Chicago
Jin, M., Park, S. Y., Shen, Q., Lai, Y., Ou, X., Mao, Z., Lin, D., Yu, Y., Zhang, W."Anti-neuroinflammatory effect of curcumin on Pam3CSK4-stimulated microglial cells". International Journal of Molecular Medicine 41, no. 1 (2018): 521-530. https://doi.org/10.3892/ijmm.2017.3217