Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation

  • Authors:
    • Qipeng Zhao
    • Ailing Chen
    • Xiaobo Wang
    • Zhuanzhuan Zhang
    • Yunsheng Zhao
    • Yu Huang
    • Shuanglai Ren
    • Yafei Zhu
  • View Affiliations

  • Published online on: May 17, 2018     https://doi.org/10.3892/ijmm.2018.3691
  • Pages: 1190-1198
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The present study aimed to investigate the protective effects of dehydrocostuslactone (DHL) against rat hippocampal slice injury caused by oxygen‑glucose deprivation/reoxygenation (OGD/R). Rat hippocampal slice injury was induced by OGD/R in vitro, and the degree of injury was evaluated through a lactate dehydrogenase (LDH) assay and 2,3,5‑triphenyltetrazolium chloride (TTC) staining. The protein expression levels of B‑cell lymphoma-2 (Bcl‑2), Bcl‑2‑associated X protein (Bax), cytochrome c (cyt‑c), apoptotic protease activating factor 1 (apaf‑1), caspase‑9, caspase‑7, caspase‑3, sequestosome 1 (SQSTM1) and microtubule‑associated protein 1 light chain 3 (LC3) were analyzed through western blot analysis. The results showed that 1, 5 and 10 µM DHL decreased the levels of LDH (P<0.05) and increased the A490 value of TTC (P<0.05). Furthermore, the expression of Bcl‑2 was enhanced, and the protein expression levels of Bax, cyt‑c, apaf‑1, caspase‑9, caspase‑7, caspase‑3, SQSTM1 and LC3 were significantly inhibited (P<0.05), compared with those in the OGD/R group. These results suggested that DHL elicited protective effects against hippocampal OGD/R injury, and its underlying mechanism may be associated with inhibiting apoptosis.
View Figures
View References

Related Articles

Journal Cover

August-2018
Volume 42 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Q, Chen A, Wang X, Zhang Z, Zhao Y, Huang Y, Ren S and Zhu Y: Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. Int J Mol Med 42: 1190-1198, 2018
APA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y. ... Zhu, Y. (2018). Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation. International Journal of Molecular Medicine, 42, 1190-1198. https://doi.org/10.3892/ijmm.2018.3691
MLA
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42.2 (2018): 1190-1198.
Chicago
Zhao, Q., Chen, A., Wang, X., Zhang, Z., Zhao, Y., Huang, Y., Ren, S., Zhu, Y."Protective effects of dehydrocostuslactone on rat hippocampal slice injury induced by oxygen‑glucose deprivation/reoxygenation". International Journal of Molecular Medicine 42, no. 2 (2018): 1190-1198. https://doi.org/10.3892/ijmm.2018.3691