Cytotoxic effects of ultra-diluted remedies on breast cancer cells

  • Authors:
    • Moshe Frenkel
    • Bal Mukund Mishra
    • Subrata Sen
    • Peiying Yang
    • Alison Pawlus
    • Luis Vence
    • Aimee Leblanc
    • Lorenzo Cohen
    • Pratip Banerji
    • Prasanta Banerji
  • View Affiliations

  • Published online on: February 1, 2010     https://doi.org/10.3892/ijo_00000512
  • Pages: 395-403
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Abstract

The use of ultra-diluted natural products in the management of disease and treatment of cancer has generated a lot of interest and controversy. We conducted an in vitro study to determine if products prescribed by a clinic in India have any effect on breast cancer cell lines. We studied four ultra-diluted remedies (Carcinosin, Phytolacca, Conium and Thuja) against two human breast adenocarcinoma cell lines (MCF-7 and MDA-MB-231) and a cell line derived from immortalized normal human mammary epithelial cells (HMLE). The remedies exerted preferential cytotoxic effects against the two breast cancer cell lines, causing cell cycle delay/arrest and apoptosis. These effects were accompanied by altered expression of the cell cycle regulatory proteins, including downregulation of phosphorylated Rb and upregulation of the CDK inhibitor p27, which were likely responsible for the cell cycle delay/arrest as well as induction of the apoptotic cascade that manifested in the activation of caspase 7 and cleavage of PARP in the treated cells. The findings demonstrate biological activity of these natural products when presented at ultra-diluted doses. Further in-depth studies with additional cell lines and animal models are warranted to explore the clinical applicability of these agents.

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February 2010
Volume 36 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Frenkel M, Mishra BM, Sen S, Yang P, Pawlus A, Vence L, Leblanc A, Cohen L, Banerji P, Banerji P, Banerji P, et al: Cytotoxic effects of ultra-diluted remedies on breast cancer cells. Int J Oncol 36: 395-403, 2010.
APA
Frenkel, M., Mishra, B.M., Sen, S., Yang, P., Pawlus, A., Vence, L. ... Banerji, P. (2010). Cytotoxic effects of ultra-diluted remedies on breast cancer cells. International Journal of Oncology, 36, 395-403. https://doi.org/10.3892/ijo_00000512
MLA
Frenkel, M., Mishra, B. M., Sen, S., Yang, P., Pawlus, A., Vence, L., Leblanc, A., Cohen, L., Banerji, P., Banerji, P."Cytotoxic effects of ultra-diluted remedies on breast cancer cells". International Journal of Oncology 36.2 (2010): 395-403.
Chicago
Frenkel, M., Mishra, B. M., Sen, S., Yang, P., Pawlus, A., Vence, L., Leblanc, A., Cohen, L., Banerji, P., Banerji, P."Cytotoxic effects of ultra-diluted remedies on breast cancer cells". International Journal of Oncology 36, no. 2 (2010): 395-403. https://doi.org/10.3892/ijo_00000512