Cyclooxygenase-2 and 5-lipoxygenase pathways in diosgenin-induced apoptosis in HT-29 and HCT-116 colon cancer cells

  • Authors:
    • Clemence Lepage
    • Bertrand Liagre
    • Jeanne Cook-Moreau
    • Aline Pinon
    • Jean-Louis Beneytout
  • View Affiliations

  • Published online on: May 1, 2010     https://doi.org/10.3892/ijo_00000601
  • Pages: 1183-1191
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Abstract

We studied the relationship between diosgenin-induced apoptosis and arachidonic acid metabolism in two cancer cell lines, the HT-29 and HCT-116. Diosgenin is a steroidal saponin known for its antiproliferative and pro-apoptotic effects on cancer cells. We focused our attention on two enzymes intervening in two different pathways of arachidonic metabolism: cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). HT-29 and HCT-116 cells express 5-LOX but only HT-29 cells express COX-2 since HCT-116 are deficient. After 40 µM diosgenin treatment, we observed apoptosis hallmarks in both cell lines but HT-29 cells were more resistant with delayed apoptosis. In these cells, COX-2 expression and activity were increased and for the first time we showed that diosgenin also increased 5-LOX expression and enhanced leukotriene B4 production. Inhibition of 5-LOX by AA-861 significantly reduced apoptosis in both cell lines but COX-2 inhibition by NS-398 strongly sensitized HT-29 cells to diosgenin-induced apoptosis compared to HCT-116 cells. In this study, we showed the implication of COX-2 and 5-LOX in diosgenin-induced apoptosis but these results demonstrate how difficult it is to assess the correlation between the apoptotic signalling pathway of diosgenin and arachidonic acid metabolism with certitude.

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May 2010
Volume 36 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Lepage C, Liagre B, Cook-Moreau J, Pinon A and Beneytout J: Cyclooxygenase-2 and 5-lipoxygenase pathways in diosgenin-induced apoptosis in HT-29 and HCT-116 colon cancer cells. Int J Oncol 36: 1183-1191, 2010
APA
Lepage, C., Liagre, B., Cook-Moreau, J., Pinon, A., & Beneytout, J. (2010). Cyclooxygenase-2 and 5-lipoxygenase pathways in diosgenin-induced apoptosis in HT-29 and HCT-116 colon cancer cells. International Journal of Oncology, 36, 1183-1191. https://doi.org/10.3892/ijo_00000601
MLA
Lepage, C., Liagre, B., Cook-Moreau, J., Pinon, A., Beneytout, J."Cyclooxygenase-2 and 5-lipoxygenase pathways in diosgenin-induced apoptosis in HT-29 and HCT-116 colon cancer cells". International Journal of Oncology 36.5 (2010): 1183-1191.
Chicago
Lepage, C., Liagre, B., Cook-Moreau, J., Pinon, A., Beneytout, J."Cyclooxygenase-2 and 5-lipoxygenase pathways in diosgenin-induced apoptosis in HT-29 and HCT-116 colon cancer cells". International Journal of Oncology 36, no. 5 (2010): 1183-1191. https://doi.org/10.3892/ijo_00000601