RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro

  • Authors:
    • Haipeng Si
    • Changliang Peng
    • Jingjing Li
    • Xiqian Wang
    • Lianwen Zhai
    • Xiaofeng Li
    • Jianmin Li
  • View Affiliations

  • Published online on: December 15, 2011     https://doi.org/10.3892/ijo.2011.1303
  • Pages: 1291-1297
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Abstract

Osteosarcoma is the most common primary malignancy of the bone. There have been some advances in surgical and chemotherapeutic strategies, but it is still a tumor with a high mortality rate in children and young adults. Mitogen-activated protein kinase/extracellular signal regulated kinase (ERK) pathway plays an essential role in the development and progression of various tumors. ERK1/2 is a key component of this pathway and hyperactivated in different tumors including osteosarcoma. This study aimed to investigate whether downregulation of ERK1/2 by siRNA (small interfering RNA) could inhibit cell proliferation and invasion and increase chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. Results showed that the downregulation of ERK1/2 expression by siRNA in human osteosarcoma cells significantly inhibited cell proliferation and invasion in vitro. Furthermore, ERK1/2 knockdown led to cell arrest in the G1/G0 phase of the cell cycle, and eventual apoptosis and chemosensitivity enhancement in tumor cells. Our data reveal that RNAi-mediated downregulation of ERK1/2 expression can lead to potent antitumor activity and chemosensitizing effects in human osteosarcoma.
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April 2012
Volume 40 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Si H, Peng C, Li J, Wang X, Zhai L, Li X and Li J: RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. Int J Oncol 40: 1291-1297, 2012
APA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., & Li, J. (2012). RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro. International Journal of Oncology, 40, 1291-1297. https://doi.org/10.3892/ijo.2011.1303
MLA
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40.4 (2012): 1291-1297.
Chicago
Si, H., Peng, C., Li, J., Wang, X., Zhai, L., Li, X., Li, J."RNAi-mediated knockdown of ERK1/2 inhibits cell proliferation and invasion and increases chemosensitivity to cisplatin in human osteosarcoma U2-OS cells in vitro". International Journal of Oncology 40, no. 4 (2012): 1291-1297. https://doi.org/10.3892/ijo.2011.1303