Open Access

Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma

  • Authors:
    • Elena Zachariades
    • Dionisis Mparmpakas
    • Peter Thomas
    • Mariann Rand-Weaver
    • Emmanouil Karteris
  • View Affiliations

  • Published online on: January 27, 2012     https://doi.org/10.3892/ijo.2012.1349
  • Pages: 1358-1364
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Abstract

Choriocarcinoma is a highly malignant epithelial tumour that is most often associated with hydatidiform mole and presents the most common emergency medical problem in the management of trophoblast disease. We hypothesise that the hormones/cytokines present within the tumour microenvironment play key roles in the development of choriocarcinoma. In this study we assessed the effects of interleukin-1β (IL-1β) on cell death in the presence or absence of the sex hormone progesterone using two choriocarcinoma cell lines (BeWo and JEG-3) as in vitro experimental models. Although IL-1β induced cell death in both cell lines, the effect was more pronounced in JEG-3 cells, where cell death reached 40% compared to 15% in BeWo cells. Cell death of JEG-3 cells in response to IL-1β was significantly decreased by co-treatment with 100 nM and 1000 nM progesterone and completely abolished at a progesterone concentration of 1000 nM. Progesterone was also able to induce phosphorylation of ERK1/2 in these cells. Pretreatment of JEG-3 cells with a specific MAPK inhibitor (UO126) inhibited progesterone's inhibitory effect on cell death. Collectively, these data provide evidence of cross-talk between progesterone and IL-1β in this aggressive and poorly understood tumour that involves activation of a MAPK pathway and involvement of numerous progesterone receptors.

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May 2012
Volume 40 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Zachariades E, Mparmpakas D, Thomas P, Rand-Weaver M and Karteris E: Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma. Int J Oncol 40: 1358-1364, 2012
APA
Zachariades, E., Mparmpakas, D., Thomas, P., Rand-Weaver, M., & Karteris, E. (2012). Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma. International Journal of Oncology, 40, 1358-1364. https://doi.org/10.3892/ijo.2012.1349
MLA
Zachariades, E., Mparmpakas, D., Thomas, P., Rand-Weaver, M., Karteris, E."Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma". International Journal of Oncology 40.5 (2012): 1358-1364.
Chicago
Zachariades, E., Mparmpakas, D., Thomas, P., Rand-Weaver, M., Karteris, E."Crucial cross-talk of interleukin-1β and progesterone in human choriocarcinoma". International Journal of Oncology 40, no. 5 (2012): 1358-1364. https://doi.org/10.3892/ijo.2012.1349