Breast cancer proteomics reveals a positive correlation between glyoxalase 1 expression and high tumor grade

Fonseca-Sánchez,Miguel  A.; Rodríguez Cuevas,Sergio; Mendoza-Hernández,Guillermo; Bautista-Piña,Veronica; Arechaga Ocampo,Elena; Hidalgo Miranda,Alfredo; Quintanar Jurado,Valeria; Marchat,Laurence   A.; Álvarez-Sánchez,Elizbeth; Pérez Plasencia,Carlos; López-Camarillo,César

doi:10.3892/ijo.2012.1478

Breast cancer proteomics reveals a positive correlation between glyoxalase 1 expression and high tumor grade

Authors:
- Miguel A. Fonseca-Sánchez
- Sergio Rodríguez Cuevas
- Guillermo Mendoza-Hernández
- Veronica Bautista-Piña
- Elena Arechaga Ocampo
- Alfredo Hidalgo Miranda
- Valeria Quintanar Jurado
- Laurence A. Marchat
- Elizbeth Álvarez-Sánchez
- Carlos Pérez Plasencia
- César López-Camarillo
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Affiliations: Genomics Sciences Program, Oncogenomics and Cancer Proteomics Laboratory, Autonomous University of Mexico city, 03100 Mexico City, Mexico, Institute of Breast Diseases-FUCAM, 04980 Mexico City, Mexico, Biochemistry Department, Medicine Faculty, Autonomous National University of Mexico, Mexico City, Mexico, Carcinogenesis Laboratory, National Institute of Cancerology, 14080 Mexico City, Mexico, National Institute of Genomic Medicine, Mexico City, Mexico, Biotechnology Program, Institutional Program of Molecular Biomedicine, National School of Medicine and Homeopathy of the National Polytechnic Institute, 07320 Mexico City, Mexico, Genomics Sciences Program, Oncogenomics and Cancer Proteomics Laboratory, Autonomous University of Mexico City, 03100 Mexico City, Mexico, Massive Sequencing Unit, National Institute of Cancerology, 14080 Mexico City, Mexico, Genomics Sciences Program, Oncogenomics and Cancer Proteomics Laboratory, Autonomous University of Mexico City, 03100 Mexico city, Mexico
Published online on: May 14, 2012 https://doi.org/10.3892/ijo.2012.1478
Pages: 670-680

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Abstract

Breast cancer is the neoplasia with the highest incidence in women worldwide. Proteomics approaches have accelerated the discovery of diagnostic and prognostic biomarkers. Here, we compared the proteomic profiles of breast tumors versus non-tumoral tissues in order to identify modulated proteins, which could represent potential markers associated to clinical features. By two-dimensional electrophoresis, we detected 28 differentially expressed proteins. Among these, 21 proteins were up-regulated and 7 were down-regulated in tumors (p<0.05). Proteins were identified using LC/ESI-MS/MS tandem mass spectrometry. One protein was identified as glyoxalase 1 (GLO1), an enzyme involved in detoxification of methylglyoxal, a cytotoxic product of glycolysis. GLO1 overexpression was confirmed by western blot assays in paired normal and tumor breast tissues in clinical stages I-III, and by immunohistochemistry on tissue microarrays (TMA) comprising a cohort of 98 breast tumors and 20 healthy specimens. Results from TMA demonstrated that GLO1 is overexpressed in 79% of tumors. Interestingly, GLO1 up-regulation correlates with advanced tumor grade (p<0.05). These findings demonstrate the association of GLO1 overexpression with tumor grade and pointed out for additional studies to establish the importance of GLO1 in breast cancer.

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