Open Access

MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells

  • Authors:
    • Mengru Cao
    • Masahiro Seike
    • Chie Soeno
    • Hideaki Mizutani
    • Kazuhiro Kitamura
    • Yuji Minegishi
    • Rintaro Noro
    • Akinobu Yoshimura
    • Li Cai
    • Akihiko Gemma
  • View Affiliations

  • Published online on: June 28, 2012     https://doi.org/10.3892/ijo.2012.1535
  • Pages: 869-875
  • Copyright: © Cao et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Transforming growth factor-β (TGF-β)-induced epithelial-mesenchymal transition (EMT) has been shown to be related to the pathogenesis of various diseases including lung cancer. Recently, microRNAs (miRNA) have been recognized as a new class of genes involved in human tumorigenesis. MiR-23a/24/27a is a miRNA cluster located in chromosome 19p13.12, which can function as an oncogene in several human cancers. In this study, we analyzed miR-23a/24/27a expression in 10 non-small cell cancer (NSCLC) cell lines by real-time PCR analysis. Correlation between expression of these miRNAs and TGF-β/Smad signaling was evaluated. We found that miR-23a could be regulated by TGF-β1 in a Smad-dependent manner in A549 lung adenocarcinoma cells showing the EMT phenomenon. Knockdown of miR-23a partially restored E-cadherin expression under conditions of TGF-β1 stimulation. In contrast, overexpression of miR-23a could suppress E-cadherin expression and stimulate EMT. Furthermore, A549 cells with overexpressed miR-23a were more resistant to gefitinib compared to the parental cells. These findings suggest that miR-23a regulates TGF-β-induced EMT by targeting E-cadherin in lung cancer cells and may be useful as a new therapeutic target in NSCLC.

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September 2012
Volume 41 Issue 3

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Copy and paste a formatted citation
APA
Cao, M., Seike, M., Soeno, C., Mizutani, H., Kitamura, K., Minegishi, Y. ... Gemma, A. (2012). MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells. International Journal of Oncology, 41, 869-875. https://doi.org/10.3892/ijo.2012.1535
MLA
Cao, M., Seike, M., Soeno, C., Mizutani, H., Kitamura, K., Minegishi, Y., Noro, R., Yoshimura, A., Cai, L., Gemma, A."MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells". International Journal of Oncology 41.3 (2012): 869-875.
Chicago
Cao, M., Seike, M., Soeno, C., Mizutani, H., Kitamura, K., Minegishi, Y., Noro, R., Yoshimura, A., Cai, L., Gemma, A."MiR-23a regulates TGF-β-induced epithelial-mesenchymal transition by targeting E-cadherin in lung cancer cells". International Journal of Oncology 41, no. 3 (2012): 869-875. https://doi.org/10.3892/ijo.2012.1535