The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer

  • Authors:
    • Yunyan Wu
    • Fuyuki Sato
    • Toshiyuki Yamada
    • Ujjal Kumar Bhawal
    • Takeshi Kawamoto
    • Katsumi Fujimoto
    • Mitsuhide Noshiro
    • Hiroko Seino
    • Satoko Morohashi
    • Kenichi Hakamada
    • Yoshimitsu Abiko
    • Yukio Kato
    • Hiroshi Kijima
  • View Affiliations

  • Published online on: July 18, 2012     https://doi.org/10.3892/ijo.2012.1559
  • Pages: 1337-1346
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Abstract

DEC1 (BHLHE40/Stra13/Sharp2) is a basic helix-loop-helix (bHLH) transcription factor that is involved in the regulation of apoptosis and cell proliferation and the response to hypoxia. Epithelial-mesenchymal transition (EMT) is an important step leading to invasion and migration of various tumor cells, and TGF-β treatment has been shown to induce cancer cells to undergo EMT. However, the significance of DEC1 in TGF-β-induced EMT remains unknown. We examined the role of DEC1 in EMT of PANC-1 cells, a human pancreatic cancer cell line. As a result, we found that DEC1 was upregulated by TGF-β in PANC-1 cells, and regulated the expression and the levels of nuclear, cytoplasmic or membrane localization of EMT-related factors, including phosphorylated Smad3 (pSmad3), snail, claudin-4 and N-cadherin. In the presence of TGF-β, DEC1 knockdown by siRNA inhibited morphological changes during EMT processes, while TGF-β induced PANC-1 cells to taken on a spindle-shaped morphology. Furthermore, a combination treatment of DEC1 expression with TGF-β was closely linked to the migration and invasion of PANC-1 cells. Immunohistochemically, DEC1 and pSmad3 were detected within pancreatic cancer tissues, whereas claudin-4 expression was weaker in the cancer tissues compared with the adjacent non-cancer pancreatic tissues. These findings suggest that DEC1 plays an important role in the regulation of these EMT-related factors in pancreatic cancer.

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October 2012
Volume 41 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Wu, Y., Sato, F., Yamada, T., Bhawal, U.K., Kawamoto, T., Fujimoto, K. ... Kijima, H. (2012). The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer. International Journal of Oncology, 41, 1337-1346. https://doi.org/10.3892/ijo.2012.1559
MLA
Wu, Y., Sato, F., Yamada, T., Bhawal, U. K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Hakamada, K., Abiko, Y., Kato, Y., Kijima, H."The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer". International Journal of Oncology 41.4 (2012): 1337-1346.
Chicago
Wu, Y., Sato, F., Yamada, T., Bhawal, U. K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Hakamada, K., Abiko, Y., Kato, Y., Kijima, H."The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer". International Journal of Oncology 41, no. 4 (2012): 1337-1346. https://doi.org/10.3892/ijo.2012.1559