Differential DNA copy number aberrations in the progression of cervical lesions to invasive cervical carcinoma

  • Authors:
    • Eun Kyeong Oh
    • Yong-Wan Kim
    • In-Wook Kim
    • Hai-Bo Liu
    • Keun-Ho Lee
    • Heung Jae Chun
    • Dong Choon Park
    • Eun-Jee Oh
    • Ah Won Lee
    • Su Mi Bae
    • Woong Shick Ahn
  • View Affiliations

  • Published online on: September 27, 2012     https://doi.org/10.3892/ijo.2012.1644
  • Pages: 2038-2046
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Abstract

Host genomic alterations in addition to human papillomavirus (HPV) are needed for cervical precursor lesions to progress to invasive cancer because only a small percentage of women infected by the virus develop disease. However, the genomic alterations during the progression of cervical lesions have not been systematically examined. The aim of this study was to identify differential genomic alterations among cervical intraepithelial neoplasia CIN1, CIN2, CIN3 and cervical squamous cell carcinoma (SCC). Genomic alterations were examined for 15 cases each of CIN1, CIN2, CIN3 and SCC by array-based comparative genomic hybridization (array CGH). The chromosomal regions showing significant differential in DNA copy number aberrations (DCNAs) among CIN1, CIN2, CIN3 and SCC were successfully identified by resampling-based t-test. The chromosomal regions of 5q35.3 and 2q14.3 showed significant DCNAs between CIN1 and CIN2, and between CIN2 and CIN3, respectively, while a significant difference in DCNAs between CIN3 and SCC was observed at 1q24.3, 3p14.1, 3p14.2, 5q13.2, 7p15.3, 7q22.1 and 13q32.3. In addition, the status of DCNAs in 1q43, 2p11.2, 6p11.2, 7p21.1, 7p14.3, 10q24.1, 13q22.3, 13q34 and 16p13.3 was conserved throughout the progression of CIN to SCC. The presence of differential and common DCNAs among CIN1, CIN2, CIN3 and SCC supports that the CIN progression may include continual clonal selection and evolution. This approach also identified 34 probe sets consistently overexpressed when amplified, suggesting an unbiased identification of candidate genes in SCC during cervical cancer progression.
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December 2012
Volume 41 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Oh EK, Kim Y, Kim I, Liu H, Lee K, Chun HJ, Park DC, Oh E, Lee AW, Bae SM, Bae SM, et al: Differential DNA copy number aberrations in the progression of cervical lesions to invasive cervical carcinoma. Int J Oncol 41: 2038-2046, 2012.
APA
Oh, E.K., Kim, Y., Kim, I., Liu, H., Lee, K., Chun, H.J. ... Ahn, W.S. (2012). Differential DNA copy number aberrations in the progression of cervical lesions to invasive cervical carcinoma. International Journal of Oncology, 41, 2038-2046. https://doi.org/10.3892/ijo.2012.1644
MLA
Oh, E. K., Kim, Y., Kim, I., Liu, H., Lee, K., Chun, H. J., Park, D. C., Oh, E., Lee, A. W., Bae, S. M., Ahn, W. S."Differential DNA copy number aberrations in the progression of cervical lesions to invasive cervical carcinoma". International Journal of Oncology 41.6 (2012): 2038-2046.
Chicago
Oh, E. K., Kim, Y., Kim, I., Liu, H., Lee, K., Chun, H. J., Park, D. C., Oh, E., Lee, A. W., Bae, S. M., Ahn, W. S."Differential DNA copy number aberrations in the progression of cervical lesions to invasive cervical carcinoma". International Journal of Oncology 41, no. 6 (2012): 2038-2046. https://doi.org/10.3892/ijo.2012.1644