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MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met

  • Authors:
    • Li-Guang Wang
    • Yang Ni
    • Ben-Hua Su
    • Xue-Ru Mu
    • Hong-Chang Shen
    • Jia-Jun Du

  • View Affiliations

  • Published online on: January 10, 2013     https://doi.org/10.3892/ijo.2013.1767
  • Pages: 957-962
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Abstract

MicroRNAs (miRNAs), as a class of naturally occurring small non-coding RNAs, play profound and pervasive roles in cancer initiation and progression. Extensive decrease in miRNA levels are frequently observed in human cancers, indicating that miRNAs may function intrinsically in tumor suppression. However, the underlying mechanisms of miRNA interactions with cellular pathways are still unclear. The expression of miR-34b in non-small cell lung cancer (NSCLC) tissues was detected using quantitative real-time PCR. The relations between miR-34b expression levels and pathological stage or lymph node metastasis were assessed using the Spearman correlation test. For in vitro studies, lung cancer cells were transfected with double stranded synthetic miRNA mimics (syn-hsa-miR-34b miScript miRNA) and scrambled controls. Immunohistochemistry was used to validate the related downstream proteins of miR-34b. The expression of miR-34b was lower in NSCLC tissues compared to that in pericarcinous tissues of lung cancer. Additionally, the Spearman correlation test showed that lower miR-34b expression was correlated with higher lymph node metastasis. In vitro gain-of-function experiments indicated that miR-34b suppressed cell proliferation by inducing cell apoptosis. IHC results showed association between lower miR-34b and overexpression of phospho-Met, p53 (phospho S392) and Mdm2. Consistent with the opposing correlation between the expression of miR-34b and lymph node metastasis in NSCLC, miR-34b may play an important role in NSCLC progression. Furthermore, miR-34b downregulates Met, with subsequent changes of downstream p53 (phospho S392) and Mdm2, and inversely p53 upregulates miR-34b in a feedback loop, which provides new insights into the roles of miR-34 family members in the regulation of signaling pathways of NSCLC.
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March 2013
Volume 42 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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  • Journal Metrics
  • Impact Factor: 5.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 11.4
  • CiteScore Rank: Q1: #40/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 1.125
  • SCImago Journal Rank (SJR): 1.105
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Spandidos Publications style
Wang L, Ni Y, Su B, Mu X, Shen H and Du J: MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. Int J Oncol 42: 957-962, 2013
APA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., & Du, J. (2013). MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met. International Journal of Oncology, 42, 957-962. https://doi.org/10.3892/ijo.2013.1767
MLA
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42.3 (2013): 957-962.
Chicago
Wang, L., Ni, Y., Su, B., Mu, X., Shen, H., Du, J."MicroRNA-34b functions as a tumor suppressor and acts as a nodal point in the feedback loop with Met". International Journal of Oncology 42, no. 3 (2013): 957-962. https://doi.org/10.3892/ijo.2013.1767