CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck

  • Authors:
    • Fa‑Yu Liu
    • Jawad Safdar
    • Zhen‑Ning Li
    • Qi‑Gen Fang
    • Xu Zhang
    • Zhong‑Fei Xu
    • Chang‑Fu Sun
  • View Affiliations

  • Published online on: September 25, 2014     https://doi.org/10.3892/ijo.2014.2674
  • Pages: 2502-2510
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Abstract

Migration and invasion of tumor cells are essential prerequisites for the formation of metastasis in malignant diseases. Previously, we have reported that CC chemokine receptor 7 (CCR7) regulates the mobility of squamous cell carcinoma of head and neck (SCCHN) cells through several pathways, such as integrin and cdc42. In this study, we investigated the connection between CCR7 and mitogen‑activated protein kinase (MAPK) family members, and their influence on cell invasion and migration in metastatic SCCHN cells. Western blotting, immunostaining and fluorescence microcopy were used to detect the protein expression and distribution of MAPKs, and the Migration assay, Matrigel invasion assay and wound‑healing assay to detect the role of MAPKs in CCR7 regulating cell mobility. To analyze the correlation between CCR7 and MAPK activity and clinicopathological factors immunohistochemical staining was emplyed. The results showed stimulation of CCL19 and the activation of CCR7 could induce ERK1/2 and JNK phosphorylation, while it had no efect on p38. After activation, ERK1/2 and JNK promoted E‑cadherin low expression and Vimentin high expression. The MAPK pathway not only mediated CCR7 induced cell migration, but also mediated invasion speed. The immunohistochemistry results showed that CCR7 was correlated with the phosphorylation of ERK1/2 and JNK in SCCHN, and these molecules were all associated with lymph node metastasis. Therefore, our study demonstrates that MAPK members (ERK1/2 and JNK) play a key role in CCR7 regulating SCCHN metastasis.
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December-2014
Volume 45 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Liu FY, Safdar J, Li ZN, Fang QG, Zhang X, Xu ZF and Sun CF: CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck. Int J Oncol 45: 2502-2510, 2014.
APA
Liu, F., Safdar, J., Li, Z., Fang, Q., Zhang, X., Xu, Z., & Sun, C. (2014). CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck. International Journal of Oncology, 45, 2502-2510. https://doi.org/10.3892/ijo.2014.2674
MLA
Liu, F., Safdar, J., Li, Z., Fang, Q., Zhang, X., Xu, Z., Sun, C."CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck". International Journal of Oncology 45.6 (2014): 2502-2510.
Chicago
Liu, F., Safdar, J., Li, Z., Fang, Q., Zhang, X., Xu, Z., Sun, C."CCR7 regulates cell migration and invasion through MAPKs in metastatic squamous cell carcinoma of head and neck". International Journal of Oncology 45, no. 6 (2014): 2502-2510. https://doi.org/10.3892/ijo.2014.2674