Celecoxib exerts antitumor effects in canine mammary tumor cells via COX‑2‑independent mechanisms

  • Authors:
    • Dai Tamura
    • Teruyoshi Saito
    • Kanae Murata
    • Masafumi Kawashima
    • Ryuji Asano
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  • Published online on: January 8, 2015     https://doi.org/10.3892/ijo.2015.2820
  • Pages: 1393-1404
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Abstract

Celecoxib plays antitumor roles via multiple mechanisms in a variety of human cancers. The aim of this study was to clarify the mechanism of action of celecoxib in canine mammary tumors. We examined the antitumor effects of celecoxib in AZACB canine mammary tumor cells expressing low levels of cyclooxygenase‑2 (COX‑2) to minimize the effect of COX‑2 on its activity. Our data revealed that celecoxib inhibited cell proliferation mainly via COX‑2‑independent mechanisms. Specifically, celecoxib decreased the proportion of cells in S phase and increased G2/M arrest, which was associated with increased expression of the cyclin‑dependent kinase inhibitors (CDKIs) p21 and p27. In addition, treatment with celecoxib downregulated COX‑2 expression, and induced apoptosis via both the intrinsic and extrinsic pathways. These findings suggest that celecoxib might be a useful agent for the treatment of canine mammary tumors, regardless of COX‑2 expression. In the future, it might be possible to use a combination of celecoxib and other antitumor agents to treat canine mammary tumors.
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March-2015
Volume 46 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Tamura D, Saito T, Murata K, Kawashima M and Asano R: Celecoxib exerts antitumor effects in canine mammary tumor cells via COX‑2‑independent mechanisms. Int J Oncol 46: 1393-1404, 2015
APA
Tamura, D., Saito, T., Murata, K., Kawashima, M., & Asano, R. (2015). Celecoxib exerts antitumor effects in canine mammary tumor cells via COX‑2‑independent mechanisms. International Journal of Oncology, 46, 1393-1404. https://doi.org/10.3892/ijo.2015.2820
MLA
Tamura, D., Saito, T., Murata, K., Kawashima, M., Asano, R."Celecoxib exerts antitumor effects in canine mammary tumor cells via COX‑2‑independent mechanisms". International Journal of Oncology 46.3 (2015): 1393-1404.
Chicago
Tamura, D., Saito, T., Murata, K., Kawashima, M., Asano, R."Celecoxib exerts antitumor effects in canine mammary tumor cells via COX‑2‑independent mechanisms". International Journal of Oncology 46, no. 3 (2015): 1393-1404. https://doi.org/10.3892/ijo.2015.2820