Constitutive expression of IRF-5 in HTLV-1-infected T cells

  • Authors:
    • Chie Ishikawa
    • Masachika Senba
    • Betsy J. Barnes
    • Naoki Mori
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  • Published online on: May 22, 2015     https://doi.org/10.3892/ijo.2015.3020
  • Pages: 361-369
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Abstract

Human T-cell leukemia virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia (ATL), an aggressive and fatal leukemia of T cells. Interferon regulatory factor (IRF)-5 plays a critical role in the induction of interferon genes in viral infected cells. We examined the specific mechanisms underlying the expression and regulation of IRF-5 in HTLV-1-infected T cells. IRF-5 was constitutively transcribed into three distinct alternatively spliced isoforms (V1, V3 and V4) in HTLV-1-infected T-cell lines but not in uninfected T-cell lines. IRF-5 was also upregulated in HTLV-1-infected T-cell lines at protein level. Nuclear IRF-5 expression was noted in ATL cells present in lymph nodes and skin lesions. IRF-5 mRNA expression was induced following infection of T cells with HTLV-1, and specifically by viral oncoprotein Tax. Tax also activated V3 promoter. Microarray analysis of IRF-5-expressing uninfected T cells demonstrated that IRF-5 induced the expression of tumor necrosis factor family cytokines. The results suggest that IRF-5 is a Tax-regulated gene, and its expression may be associated with the pathogenesis of ATL.
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July-2015
Volume 47 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Ishikawa C, Senba M, Barnes BJ and Mori N: Constitutive expression of IRF-5 in HTLV-1-infected T cells. Int J Oncol 47: 361-369, 2015
APA
Ishikawa, C., Senba, M., Barnes, B.J., & Mori, N. (2015). Constitutive expression of IRF-5 in HTLV-1-infected T cells. International Journal of Oncology, 47, 361-369. https://doi.org/10.3892/ijo.2015.3020
MLA
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47.1 (2015): 361-369.
Chicago
Ishikawa, C., Senba, M., Barnes, B. J., Mori, N."Constitutive expression of IRF-5 in HTLV-1-infected T cells". International Journal of Oncology 47, no. 1 (2015): 361-369. https://doi.org/10.3892/ijo.2015.3020