Identification of miR-15b as a transformation-related factor in mantle cell lymphoma

Arakawa,Fumiko; Kimura,Yoshizo; Yoshida,Noriaki; Miyoshi,Hiroaki; Doi,Atushi; Yasuda,Kaori; Nakajima,Kazutaka; Kiyasu,Junichi; Niino,Daisuke; Sugita,Yasuo; Tashiro,Kosuke; Kuhara,Satoru; Seto,Masao; Ohshima,Koichi

doi:10.3892/ijo.2015.3295

Identification of miR-15b as a transformation-related factor in mantle cell lymphoma

Authors:
- Fumiko Arakawa
- Yoshizo Kimura
- Noriaki Yoshida
- Hiroaki Miyoshi
- Atushi Doi
- Kaori Yasuda
- Kazutaka Nakajima
- Junichi Kiyasu
- Daisuke Niino
- Yasuo Sugita
- Kosuke Tashiro
- Satoru Kuhara
- Masao Seto
- Koichi Ohshima
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Affiliations: Department of Pathology, School of Medicine, Kurume University, Kurume, Japan, Cell Innovator Inc., Venture Business Laboratory of Kyushu University, Fukuoka, Japan, Laboratory of Molecular Gene Technology and Molecular Biosciences, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
Published online on: December 15, 2015 https://doi.org/10.3892/ijo.2015.3295
Pages: 485-492
Copyright: © Arakawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Mantle cell lymphoma (MCL) is an aggressive B cell lymphoma with a poor prognosis. It is characterized by the t(11;14)(q13;q32) translocation, resulting in overexpression of CCND1. Morphologically, MCL is categorised into two types: classical MCL (cMCL) and aggressive MCL (aMCL), with a proportion of cMCL progressing to develop into aMCL. miRNAs are currently considered to be important regulators for cell behavior and are deregulated in many malignancies. Although several genetic alterations have been implicated in the transformation of cMCL to aMCL, the involvement of miRNAs in transformation is not known. In an effort to identify the miRNAs related to the transformation of MCL, miRNA microarray analyses were used for cMCL and aMCL cases. These analyses demonstrated significant differences in the expression of seven microRNAs based on a t-test (p-value <0.05); miR-15b was greatly upregulated in aMCL. Locked nucleic acid in situ hybridization showed increased staining of miR-15b in formalin-fixed paraffin-embedded sections of aMCL. These results correlated well with the microRNA microarray analysis. Although the molecular functions of miR-15b are largely unknown, it has been found to be associated with the cell cycle and apoptosis. However, the physiological significance of increased miR-15b in MCL is still unknown. Our present findings suggest that the upregulated expression of miR-15b is likely to play an important role in the transformation of cMCL to aMCL.

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