Open Access

MicroRNA analysis of breast ductal fluid in breast cancer patients

  • Authors:
    • Luisa Matos Do Canto
    • Catalin Marian
    • Shawna Willey
    • Mary Sidawy
    • Patricia A. Da Cunha
    • Janice D. Rone
    • Xin Li
    • Yuriy Gusev
    • Bassem R. Haddad
  • View Affiliations

  • Published online on: March 10, 2016     https://doi.org/10.3892/ijo.2016.3435
  • Pages: 2071-2078
  • Copyright: © Do Canto et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Recent studies suggest that microRNAs show promise as excellent biomarkers for breast cancer; however there is still a high degree of variability between studies making the findings difficult to interpret. In addition to blood, ductal lavage (DL) and nipple aspirate fluids represent an excellent opportunity for biomarker detection because they can be obtained in a less invasive manner than biopsies and circumvent the limitations of evaluating blood biomarkers with regards to tissue of origin specificity. In this study, we have investigated for the first time, through a real-time PCR array, the expression of 742 miRNAs in the ductal lavage fluid collected from 22 women with unilateral breast tumors. We identified 17 differentially expressed miRNAs between tumor and paired normal samples from patients with ductal breast carcinoma. Most of these miRNAs have various roles in breast cancer tumorigenesis, invasion and metastasis, therapeutic response, or are associated with several clinical and pathological characteristics of breast tumors. Moreover, some miRNAs were also detected in other biological fluids of breast cancer patients such as serum (miR-23b, -133b, -181a, 338-3p, -625), plasma (miR-200a), and breast milk (miR-181a). A systems biology analysis of these differentially expressed miRNAs points out possible pathways and cellular processes previously described as having an important role in breast cancer such as Wnt, ErbB, MAPK, TGF-β, mTOR, PI3K-Akt, p53 signaling pathways. We also observed a difference in the miRNA expression with respect to the histological type of the tumors. In conclusion, our findings suggest that miRNA analysis of breast ductal fluid is feasible and potentially very useful for the detection of breast cancer.
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May-2016
Volume 48 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Do Canto LM, Marian C, Willey S, Sidawy M, Da Cunha PA, Rone JD, Li X, Gusev Y and Haddad BR: MicroRNA analysis of breast ductal fluid in breast cancer patients. Int J Oncol 48: 2071-2078, 2016
APA
Do Canto, L.M., Marian, C., Willey, S., Sidawy, M., Da Cunha, P.A., Rone, J.D. ... Haddad, B.R. (2016). MicroRNA analysis of breast ductal fluid in breast cancer patients. International Journal of Oncology, 48, 2071-2078. https://doi.org/10.3892/ijo.2016.3435
MLA
Do Canto, L. M., Marian, C., Willey, S., Sidawy, M., Da Cunha, P. A., Rone, J. D., Li, X., Gusev, Y., Haddad, B. R."MicroRNA analysis of breast ductal fluid in breast cancer patients". International Journal of Oncology 48.5 (2016): 2071-2078.
Chicago
Do Canto, L. M., Marian, C., Willey, S., Sidawy, M., Da Cunha, P. A., Rone, J. D., Li, X., Gusev, Y., Haddad, B. R."MicroRNA analysis of breast ductal fluid in breast cancer patients". International Journal of Oncology 48, no. 5 (2016): 2071-2078. https://doi.org/10.3892/ijo.2016.3435