miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2

  • Authors:
    • Norahayu Othman
    • Noor Hasima Nagoor
  • View Affiliations

  • Published online on: October 23, 2017     https://doi.org/10.3892/ijo.2017.4174
  • Pages:1757-1764
0

Abstract

Lung cancer remains a major health problem with a low 5-year survival rate of patients. Recent studies have shown that dysregulation of microRNAs (miRNAs) are prevalent in lung cancer and these aberrations play a significant role in the progression of tumour progression. In the present study, bioinformatics analyses was employed to predict potential miR-608 targets, which are associated with signaling pathways involved in cancer. Luciferase reporter assay identified AKT2 as a novel target of miR-608, and suppression of its protein levels was validated through western blot analysis. Zebrafish embryos were microinjected with cells transfected with miR-608 to elucidate the role of miR-608 in vivo, and immunostained with antibodies to detect activated caspase-3. We present the first evidence that miR-608 behaves as a tumour suppressor in A549 and SK-LU-1 cells through the regulation of AKT2, suggesting that selective targeting of AKT2 via miR-608 may be developed as a potential therapeutic strategy for miRNA-based non-small cell lung cancer (NSCLC) therapy.

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December 2017
Volume 51 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2016 Impact Factor: 3.079
Ranked #33/217 Oncology
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APA
Othman, N., & Othman, N. (2017). miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2. International Journal of Oncology, 51, 1757-1764. https://doi.org/10.3892/ijo.2017.4174
MLA
Othman, N., Nagoor, N. H."miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2". International Journal of Oncology 51.6 (2017): 1757-1764.
Chicago
Othman, N., Nagoor, N. H."miR-608 regulates apoptosis in human lung adenocarcinoma via regulation of AKT2". International Journal of Oncology 51, no. 6 (2017): 1757-1764. https://doi.org/10.3892/ijo.2017.4174