Open Access

Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer

  • Authors:
    • Bingjun Bai
    • Binbin Xie
    • Zongyou Pan
    • Lina Shan
    • Jianpei Zhao
    • Hongbo Zhu
  • View Affiliations

  • Published online on: February 22, 2018     https://doi.org/10.3892/ijo.2018.4281
  • Pages: 1129-1138
  • Copyright: © Bai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The incidence and development of colorectal cancer (CRC) is a process with multiple gene interactions. We have previously demonstrated that ATP synthase-coupling factor 6, mitochondrial (ATP5J) is associated with CRC migration and 5-fluorouracil resistance; nevertheless, the exact molecular mechanism remains unclear. The following study uses microarray and bioinformatics methods to identify candidate genes and long non-coding RNAs (lncRNAs) in CRC cells (two pairs) with upregulated and downregulated ATP5J. Briefly, a total of 2,190 differentially expressed mRNAs (DEmRNAs) were sorted. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed for 4 DEmRNAs to validate the results of microarray analysis. Functional annotation and pathway enrichment were analyzed for DEmRNAs using the Database for Annotation, Visualization and Integrated Discovery. Significantly enriched pathways included the regulation of gene expression and cell growth. The protein‑protein interaction network was constructed, and AKT serine/threonine kinase 2 (AKT2) was considered as one of the hub genes. For further analysis, 51 DEmRNAs and 30 DElncRNAs were selected that were positively or negatively associated with the expression of ATP5J in the two cell pairs. X-inactive specific transcript (XIST), premature ovarian failure 1B (POF1B) and calmin (CLMN) were identified in the DEmRNA-DElncRNA co-expression network. The expression of AKT2 and XIST in CRC cells was confirmed by RT-qPCR. To sum up, the candidate genes and lncRNAs, as well as potential signaling pathways, which were identified using integrated bioinformatics analysis, could improve the understanding of molecular events involved in the function of ATP5J in CRC.
View Figures
View References

Related Articles

Journal Cover

April-2018
Volume 52 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Bai B, Xie B, Pan Z, Shan L, Zhao J and Zhu H: Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer. Int J Oncol 52: 1129-1138, 2018.
APA
Bai, B., Xie, B., Pan, Z., Shan, L., Zhao, J., & Zhu, H. (2018). Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer. International Journal of Oncology, 52, 1129-1138. https://doi.org/10.3892/ijo.2018.4281
MLA
Bai, B., Xie, B., Pan, Z., Shan, L., Zhao, J., Zhu, H."Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer". International Journal of Oncology 52.4 (2018): 1129-1138.
Chicago
Bai, B., Xie, B., Pan, Z., Shan, L., Zhao, J., Zhu, H."Identification of candidate genes and long non-coding RNAs associated with the effect of ATP5J in colorectal cancer". International Journal of Oncology 52, no. 4 (2018): 1129-1138. https://doi.org/10.3892/ijo.2018.4281