Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro

  • Authors:
    • Xiaoyi Chen
    • Zhijie Xu
    • Zhijian Zhu
    • Anqi Chen
    • Guanghou Fu
    • Yimin Wang
    • Hao Pan
    • Baiye Jin
  • View Affiliations

  • Published online on: July 25, 2018     https://doi.org/10.3892/ijo.2018.4501
  • Pages: 1703-1712
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Glucose-6-phosphate dehydrogenase (G6PD) is a rate-limiting enzyme of the pentose phosphate pathway. Multiple studies have previously revealed that elevated G6PD levels promote cancer progression in numerous tumor types; however, the underlying mechanism remains unclear. In the present study, it was demonstrated that high G6PD expression is a poor prognostic factor in bladder cancer, and the levels of G6PD expression increase with increasing tumor stage. Patients with bladder cancer with high G6PD expression had worse survival rates compared with those with lower G6PD expression in resected tumors. In vitro experiments revealed that knockdown of G6PD suppressed cell viability and growth in Cell Counting Kit-8 and colony formation assays, and increased apoptosis in bladder cancer cell lines compared with normal cells. Further experiments indicated that the weakening of the survival ability in G6PD-knockdown bladder cancer cells may be explained by intracellular reactive oxygen species accumulation and protein kinase B pathway suppression. Furthermore, it was additionally revealed that 6-aminonicotinamide (6-AN), a competitive G6PD inhibitor, may be a potential therapy for bladder cancer, particularly in cases with high G6PD expression, and that the combination of cisplatin and 6-AN may optimize the clinical dose or minimize the side effects of cisplatin.
View Figures
View References

Related Articles

Journal Cover

October-2018
Volume 53 Issue 4

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chen X, Xu Z, Zhu Z, Chen A, Fu G, Wang Y, Pan H and Jin B: Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro. Int J Oncol 53: 1703-1712, 2018
APA
Chen, X., Xu, Z., Zhu, Z., Chen, A., Fu, G., Wang, Y. ... Jin, B. (2018). Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro. International Journal of Oncology, 53, 1703-1712. https://doi.org/10.3892/ijo.2018.4501
MLA
Chen, X., Xu, Z., Zhu, Z., Chen, A., Fu, G., Wang, Y., Pan, H., Jin, B."Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro". International Journal of Oncology 53.4 (2018): 1703-1712.
Chicago
Chen, X., Xu, Z., Zhu, Z., Chen, A., Fu, G., Wang, Y., Pan, H., Jin, B."Modulation of G6PD affects bladder cancer via ROS accumulation and the AKT pathway in vitro". International Journal of Oncology 53, no. 4 (2018): 1703-1712. https://doi.org/10.3892/ijo.2018.4501