Open Access

KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer

  • Authors:
    • Zao-Zao Wang
    • Jie Yang
    • Bei-Hai Jiang
    • Jia-Bo Di
    • Pin Gao
    • Lin Peng
    • Xiang-Qian Su
  • View Affiliations

  • Published online on: August 29, 2018     https://doi.org/10.3892/ijo.2018.4546
  • Pages: 1939-1952
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

As a mitotic kinesin, kinesin family member 14 (KIF14) has been reported to serve oncogenic roles in a variety of malignancies; however, its functional role and regulatory mechanisms in colorectal cancer (CRC) remain unclear. In the present study, KIF14 was observed to be markedly overexpressed in CRC, and this upregulation was associated with tumor size and marker of proliferation Ki-67 immunostaining scores. Gain- and loss-of-function experiments were applied to identify the function of KIF14 in CRC progression. In vitro and in vivo assays revealed that KIF14 promoted CRC cell proliferation and accelerated the cell cycle via activation of protein kinase B. In addition, the present study investigated the potential mechanisms underlying KIF14 overexpression in CRC. Bioinformatics analyses and validation experiments, including reverse transcription-quantitative polymerase chain reaction, western blotting and a Dual-Luciferase reporter assay, demonstrated that, in addition to genomic amplification and transcriptional activation, KIF14 was regulated by microRNA (miR)-200c at the post-transcriptional level. Rescue experiments further demonstrated that decreased miR-200c expression could facilitate KIF14 to exert its pro-proliferative role. The expression of miR-200c was negatively correlated with KIF14 in CRC specimens. Collectively, the findings of the present study demonstrated the oncogenic role of KIF14 in colorectal tumorigenesis, and also revealed a complexity of regulatory mechanisms mediating KIF14 overexpression, which may provide insight for developing novel treatments for patients with CRC.
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November-2018
Volume 53 Issue 5

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Spandidos Publications style
Wang Z, Yang J, Jiang B, Di J, Gao P, Peng L and Su X: KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer. Int J Oncol 53: 1939-1952, 2018
APA
Wang, Z., Yang, J., Jiang, B., Di, J., Gao, P., Peng, L., & Su, X. (2018). KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer. International Journal of Oncology, 53, 1939-1952. https://doi.org/10.3892/ijo.2018.4546
MLA
Wang, Z., Yang, J., Jiang, B., Di, J., Gao, P., Peng, L., Su, X."KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer". International Journal of Oncology 53.5 (2018): 1939-1952.
Chicago
Wang, Z., Yang, J., Jiang, B., Di, J., Gao, P., Peng, L., Su, X."KIF14 promotes cell proliferation via activation of Akt and is directly targeted by miR-200c in colorectal cancer". International Journal of Oncology 53, no. 5 (2018): 1939-1952. https://doi.org/10.3892/ijo.2018.4546