Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids

  • Authors:
    • Denise Sievers
    • Jens Bunzendahl
    • Alice Frosch
    • Christina Perske
    • Bernhard Hemmerlein
    • Henning Schliephake
    • Phillipp Brockmeyer
  • View Affiliations

  • Published online on: November 27, 2017     https://doi.org/10.3892/mco.2017.1514
  • Pages:323-325
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Abstract

Three‑dimensional (3D) multicellular spheroids (MCS) are considered suitable models in cancer research and anticancer drug development. Although studying the complex tumour characteristics from all different degrees of malignancy is vital, MCS generation has only been described in a few moderately‑ and poorly differentiated oral squamous cell carcinoma (OSCC) cell lines. No previous study has demonstrated the MCS formation in a highly differentiated OSCC cell line. For the first time, the present study aimed to generate MCS from the highly differentiated OSCC cell line BHY. BHY spheroids were grown in three independent experiments in 96‑well plates through the use of the liquid overlay technique. Although BHY cells are grow slowly and are difficult to culture, they formed compact MCS within 24 h. After 3 days of incubation, no further increase in spheroid size was observed. MCS were harvested, paraffin‑embedded and 2 µm tissue sections were prepared for further analysis. The diameter and volume of each spheroid were determined. BHY MCS diameter ranged between 46.76 and 233.26 µm, with a volume range from 5.35x104‑6.65x106 µm³. In conclusion, using the liquid overlay technique, the highly differentiated OSCC cell line BHY forms different sized spheroids, which may be used for further investigations.

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February 2018
Volume 8 Issue 2

Print ISSN: 2049-9450
Online ISSN:2049-9469

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APA
Sievers, D., Bunzendahl, J., Frosch, A., Perske, C., Hemmerlein, B., Schliephake, H., & Brockmeyer, P. (2018). Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids. Molecular and Clinical Oncology, 8, 323-325. https://doi.org/10.3892/mco.2017.1514
MLA
Sievers, D., Bunzendahl, J., Frosch, A., Perske, C., Hemmerlein, B., Schliephake, H., Brockmeyer, P."Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids". Molecular and Clinical Oncology 8.2 (2018): 323-325.
Chicago
Sievers, D., Bunzendahl, J., Frosch, A., Perske, C., Hemmerlein, B., Schliephake, H., Brockmeyer, P."Generation of highly differentiated BHY oral squamous cell carcinoma multicellular spheroids". Molecular and Clinical Oncology 8, no. 2 (2018): 323-325. https://doi.org/10.3892/mco.2017.1514