αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation

Tsujimoto,Masanori; Doi,Tomoaki; Kuroyanagi,Gen; Yamamoto,Naohiro; Matsushima‑Nishiwaki,Rie; Iida,Yuko; Enomoto,Yukiko; Iida,Hiroki; Ogura,Shinji; Otsuka,Takanobu; Tokuda,Haruhiko; Kozawa,Osamu; Iwama,Toru

doi:10.3892/mmr.2015.3445

αB-crystallin reduces ristocetin‑induced soluble CD40 ligand release in human platelets: Suppression of thromboxane A2 generation

Authors:
- Masanori Tsujimoto
- Tomoaki Doi
- Gen Kuroyanagi
- Naohiro Yamamoto
- Rie Matsushima‑Nishiwaki
- Yuko Iida
- Yukiko Enomoto
- Hiroki Iida
- Shinji Ogura
- Takanobu Otsuka
- Haruhiko Tokuda
- Osamu Kozawa
- Toru Iwama
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Affiliations: Department of Neurosugery, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Emergency and Disaster Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Pharmacology, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Anesthesiology and Pain Medicine, Gifu University Graduate School of Medicine, Gifu, Gifu 501‑1194, Japan, Department of Orthopedic Surgery, Nagoya University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8603, Japan
Published online on: March 6, 2015 https://doi.org/10.3892/mmr.2015.3445
Pages: 357-362

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Abstract

Our group has previously shown that αB‑crystallin (HSPB5), a small heat shock protein, inhibits human platelet aggregation by ristocetin, an activator of glycoprotein Ib/IX/V. In addition, it was demonstrated that glycoprotein Ib/IX/V activation induces soluble CD40 (sCD40) ligand release via thromboxane (TX) A2. In the present study, the effect of αB‑crystallin on the ristocetin‑induced sCD40 ligand release in human platelets was investigated. The ristocetin‑induced release of sCD40 ligand was suppressed by αB‑crystallin. In addition, αB‑crystallin reduced the ristocetin‑stimulated production of 11‑dehydro‑TX B2, a stable metabolite of TXA2. αB‑crystallin did not suppress the platelet aggregation induced by U46619, a TXA2 receptor agonist. αB‑crystallin had little effect on the U46619‑induced phosphorylation of p38 mitogen‑activated protein kinase or sCD40 ligand release. In addition, αB‑crystallin failed to reduce the binding of SZ2, a monoclonal antibody against the sulfated sequence in the α‑chain of glycoprotein Ib, to the ristocetin‑stimulated platelets. These results strongly suggest that αB‑crystallin extracellularly suppresses ristocetin‑stimulated release of sCD40 ligand by inhibiting the TXA2 production in human platelets.

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