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Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity

  • Authors:
    • Tanis Hogg
    • Jameson T. Mendel
    • Jonathan L. Lavezo
  • View Affiliations / Copyright

    Affiliations: Department of Medical Education, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA
    Copyright: © Hogg et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5737-5745
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    Published online on: July 29, 2015
       https://doi.org/10.3892/mmr.2015.4146
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Abstract

Pokeweed antiviral protein (PAP) belongs to the family of type I ribosome‑inactivating proteins (RIPs): Ribotoxins, which function by depurinating the sarcin‑ricin loop of ribosomal RNA. In addition to its antibacterial and antifungal properties, PAP has shown promise in antiviral and targeted tumor therapy owing to its ability to depurinate viral RNA and eukaryotic rRNA. Several PAP genes are differentially expressed across pokeweed tissues, with natively isolated seed forms of PAP exhibiting the greatest cytotoxicity. To help elucidate the molecular basis of increased cytotoxicity of PAP isoenzymes from seeds, the present study used protein sequencing, mass spectroscopy and X-ray crystallography to determine the complete covalent structure and 1.7 Å X‑ray crystal structure of PAP‑S1aci isolated from seeds of Asian pokeweed (Phytolacca acinosa). PAP‑S1aci shares ~95% sequence identity with PAP‑S1 from P. americana and contains the signature catalytic residues of the RIP superfamily, corresponding to Tyr72, Tyr122, Glu175 and Arg178 in PAP‑S1aci. A rare proline substitution (Pro174) was identified in the active site of PAP‑S1aci, which has no effect on catalytic Glu175 positioning or overall active‑site topology, yet appears to come at the expense of strained main‑chain geometry at the pre‑proline residue Val173. Notably, a rare type of N‑glycosylation was detected consisting of N‑acetyl‑D‑glucosamine monosaccharide residues linked to Asn10, Asn44 and Asn255 of PAP‑S1aci. Of note, our modeling studies suggested that the ribosome depurination activity of seed PAPs would be adversely affected by the N‑glycosylation of Asn44 and Asn255 with larger and more typical oligosaccharide chains, as they would shield the rRNA‑binding sites on the protein. These results, coupled with evidence gathered from the literature, suggest that this type of minimal N‑glycosylation in seed PAPs and other type I seed RIPs may serve to enhance cytotoxicity by exploiting receptor‑mediated uptake pathways of seed predators while preserving ribosome affinity and rRNA recognition.
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1 

Puri M, Kaur I, Perugini MA and Gupta RC: Ribosome-inactivating proteins: current status and biomedical applications. Drug Discov Today. 17:774–783. 2012. View Article : Google Scholar : PubMed/NCBI

2 

Stirpe F: Ribosome-inactivating proteins: from toxins to useful proteins. Toxicon. 67:12–16. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Endo Y, Tsurugi K and Lambert JM: The site of action of six different ribosome-inactivating proteins from plants on eukaryotic ribosomes: the RNA N-glycosidase activity of the proteins. Biochem Biophys Res Commun. 150:1032–1036. 1988. View Article : Google Scholar : PubMed/NCBI

4 

Gessner SL and Irvin JD: Inhibition of elongation factor 2-dependent translocation by the pokeweed antiviral protein and ricin. J Biol Chem. 255:3251–3253. 1980.PubMed/NCBI

5 

Stirpe F and Battelli MG: Ribosome-inactivating proteins: progress and problems. Cell Mol Life Sci. 63:1850–1866. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Rajamohan F, Kurinov IV, Venkatachalam TK and Uckun FM: Deguanylation of human immunodeficiency virus (HIV-1) RNA by recombinant pokeweed antiviral protein. Biochem Biophys Res Commun. 263:419–424. 1999. View Article : Google Scholar : PubMed/NCBI

7 

Hudak KA, Bauman JD and Tumer NE: Pokeweed antiviral protein binds to the cap structure of eukaryotic mRNA and depurinates the mRNA downstream of the cap. RNA. 8:1148–1159. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Parikh BA, Coetzer C and Tumer NE: Pokeweed antiviral protein regulates the stability of its own mRNA by a mechanism that requires depurination but can be separated from depurination of the alpha-sarcin/ricin loop of rRNA. J Biol Chem. 277:41428–41437. 2002. View Article : Google Scholar : PubMed/NCBI

9 

Parikh BA and Tumer NE: Antiviral activity of ribosome inactivating proteins in medicine. Mini Rev Med Chem. 4:523–543. 2004. View Article : Google Scholar : PubMed/NCBI

10 

Mansouri S, Choudhary G, Sarzala PM, Ratner L and Hudak KA: Suppression of human T-cell leukemia virus I gene expression by pokeweed antiviral protein. J Biol Chem. 284:31453–31462. 2009. View Article : Google Scholar : PubMed/NCBI

11 

D'Cruz OJ, Waurzyniakt B and Uckun FM: A 13-week subchronic intravaginal toxicity study of pokeweed antiviral protein in mice. Phytomedicine. 11:342–351. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Uckun FM, Rustamova L, Vassilev AO, Tibbles HE and Petkevich AS: CNS activity of pokeweed anti-viral protein (PAP) in mice infected with lymphocytic choriomeningitis virus (LCMV). BMC Infect Dis. 5:92005. View Article : Google Scholar : PubMed/NCBI

13 

Shapira A and Benhar I: Toxin-based therapeutic approaches. Toxins (Basel). 2:2519–2583. 2010. View Article : Google Scholar

14 

Benigni F, Canevari S, Gadina M, Adobati E, Ferreri AJ, Di Celle EF, Comolli R and Colnaghi MI: Preclinical evaluation of the ribosome-inactivating proteins PAP-1, PAP-S and RTA in mice. Int J Immunopharmacol. 17:829–839. 1995. View Article : Google Scholar : PubMed/NCBI

15 

Monzingo AF, Collins EJ, Ernst SR, Irvin JD and Robertus JD: The 2.5 A structure of pokeweed antiviral protein. J Mol Biol. 233:705–715. 1993. View Article : Google Scholar : PubMed/NCBI

16 

Kurinov IV, Myers DE, Irvin JD and Uckun FM: X-ray crystallographic analysis of the structural basis for the interactions of pokeweed antiviral protein with its active site inhibitor and ribosomal RNA substrate analogs. Protein Sci. 8:1765–1772. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Zeng ZH, He XL, Li HM, Hu Z and Wang DC: Crystal structure of pokeweed antiviral protein with well-defined sugars from seeds at 1.8 A resolution. J Struct Biol. 141:171–178. 2003. View Article : Google Scholar : PubMed/NCBI

18 

Hogg T, Kuta Smatanova I, Bezouska K, Ulbrich N and Hilgenfeld R: Sugar-mediated lattice contacts in crystals of a plant glycoprotein. Acta Crystallogr D Biol Crystallogr. 58:1734–1739. 2002. View Article : Google Scholar : PubMed/NCBI

19 

Brune DC: Alkylation of cysteine with acrylamide for protein sequence analysis. Anal Biochem. 207:285–290. 1992. View Article : Google Scholar : PubMed/NCBI

20 

Perkins DN, Pappin DJ, Creasy DM and Cottrell JS: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis. 20:3551–3567. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Read RJ: Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Cryst A. 42:140–149. 1986. View Article : Google Scholar

22 

Emsley P and Cowtan K: Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr. 60:2126–2132. 2004. View Article : Google Scholar : PubMed/NCBI

23 

Brünger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges M, Pannu NS, et al: Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr. 54:905–921. 1998. View Article : Google Scholar

24 

Lovell SC, Davis IW, Arendall WB 3rd, de Bakker PI, Word JM, Prisant MG, Richardson JS and Richardson DC: Structure validation by Calpha geometry: phi, psi and Cbeta deviation. Proteins. 50:437–450. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Kabsch W and Sander C: Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features. Biopolymers. 22:2577–2637. 1983. View Article : Google Scholar : PubMed/NCBI

26 

Kabsch W: A solution for the best rotation to relate two sets of vectors. Acta Cryst A. 32:922–923. 1976. View Article : Google Scholar

27 

Collaborative Computational Project, Number 4: The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr. 50:760–763. 1994. View Article : Google Scholar : PubMed/NCBI

28 

DeLano WL: The PyMOL Molecular Graphics System. DeLano Scientific; Palo Alto, USA: 2002, http://www.pymol.org.

29 

McGinnis S and Madden TL: BLAST: at the core of a powerful and diverse set of sequence analysis tools. Nucleic Acids Res. 32:W20–W25. 2004. View Article : Google Scholar : PubMed/NCBI

30 

Honjo E, Dong D, Motoshima H and Watanabe K: Genomic clones encoding two isoforms of pokeweed antiviral protein in seeds (PAP-S1 and S2) and the N-glycosidase activities of their recombinant proteins on ribosomes and DNA in comparison with other isoforms. J Biochem. 131:225–231. 2002. View Article : Google Scholar : PubMed/NCBI

31 

Corpet F: Multiple sequence alignment with hierarchical clustering. Nucl Acids Res. 16:10881–10890. 1988. View Article : Google Scholar : PubMed/NCBI

32 

Gouet P, Courcelle E, Stuart DI and Métoz F: ESPript: analysis of multiple sequence alignments in PostScript. Bioinformatics. 15:305–308. 1999. View Article : Google Scholar : PubMed/NCBI

33 

Rajamohan F, Pugmire MJ, Kurinov IV and Uckun FM: Modeling and alanine scanning mutagenesis studies of recombinant pokeweed antiviral protein. J Biol Chem. 275:3382–3390. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Altschul SF, Gish W, Miller W, Myers EW and Lipman DJ: Basic local alignment search tool. J Mol Biol. 215:403–410. 1990. View Article : Google Scholar : PubMed/NCBI

35 

Hogg T and Hilgenfeld R: Protein crystallography in drug discovery. Comprehensive Medicinal Chemistry II. Taylor JB, Triggle DJ and Kubinyi H: 3. 2nd edition. Elsevier; Amsterdam: pp. 875–900. 2007, View Article : Google Scholar

36 

Ho BK and Brasseur R: The Ramachandran plots of glycine and pre-proline. BMC Struct Biol. 5:142005. View Article : Google Scholar : PubMed/NCBI

37 

Hase S, Fujimura K, Kanoh M and Ikenaka T: Studies on heterogeneity of Taka-amylase A: isolation of an amylase having one N-acetylglucosamine residue as the sugar chain. J Biochem. 92:265–270. 1982.PubMed/NCBI

38 

Chalkley RJ, Thalhammer A, Schoepfer R and Burlingame AL: Identification of protein O-GlcNAcylation sites using electron transfer dissociation mass spectrometry on native peptides. Proc Natl Acad Sci USA. 106:8894–8899. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Wang Z, Udeshi ND, Slawson C, Compton PD, Sakabe K, Cheung WD, Shabanowitz J, Hunt DF and Hart GW: Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis. Sci Signal. 3:ra22010.PubMed/NCBI

40 

Kim YC, Jahren N, Stone MD, Udeshi ND, Markowski TW, Witthuhn BA, Shabanowitz J, Hunt DF and Olszewski NE: Identification and origin of N-linked β-D-N-acetylglucosamine monosaccharide modifications on Arabidopsis proteins. Plant Physiol. 161:455–464. 2013. View Article : Google Scholar :

41 

Islam MR, Kung SS, Kimura Y and Funatsu G: N-acetyl-D-glu cosamine-asparagine structure in ribosome-inactivating proteins from the seeds of Luffa cylindrica and Phytolacca americana. Agric Biol Chem. 55:1375–1381. 1991. View Article : Google Scholar : PubMed/NCBI

42 

Wells L, Whelan SA and Hart GW: O-GlcNAc: a regulatory post-translational modification. Biochem Biophys Res Commun. 302:435–441. 2003. View Article : Google Scholar : PubMed/NCBI

43 

Khalkhall Z and Marshall RD: Glycosylation of ribonuclease A catalysed by rabbit liver extracts. Biochem J. 146:299–307. 1975. View Article : Google Scholar : PubMed/NCBI

44 

Khalkhali Z and Marshall RD: UDP-N-acetyl-D-glucosamine-as paragine sequon N-acetyl-b-D-glucosaminyl-transferase-activity in human serum. Carbohydr Res. 49:455–473. 1976. View Article : Google Scholar : PubMed/NCBI

45 

Khalkhali Z, Marshall RD, Reuvers F, Habets-Willems C and Boer P: Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes. Biochem J. 160:37–41. 1976. View Article : Google Scholar : PubMed/NCBI

46 

Rajamohan F, Mao C and Uckun FM: Binding interactions between the active center cleft of recombinant pokeweed antiviral protein and the alpha-sarcin/ricin stem loop of ribosomal RNA. J Biol Chem. 276:24075–24081. 2001. View Article : Google Scholar : PubMed/NCBI

47 

Bonness MS, Ready MP, Irvin JD and Mabry TJ: Pokeweed antiviral protein inactivates pokeweed ribosomes; implications for the antiviral mechanism. Plant J. 5:173–183. 1994. View Article : Google Scholar : PubMed/NCBI

48 

Poyet JL and Hoeveler A: cDNA cloning and expression of pokeweed antiviral protein from seeds in Escherichia coli and its inhibition of protein synthesis in vitro. FEBS Lett. 406:97–100. 1997. View Article : Google Scholar : PubMed/NCBI

49 

Barbieri L, Battelli MG and Stirpe F: Ribosome-inactivating proteins from plants. Biochem Biophys Acta. 1154:237–282. 1993.PubMed/NCBI

50 

Gross V, Heinrich PC, vom Berq D, Steube K, Andus T, Tran-Thi TA, Decker K and Gerok W: Involvement of various organs in the initial plasma clearance of differently glycosylated rat liver secretory proteins. Eur J Biochem. 173:653–659. 1988. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hogg T, Mendel JT and Lavezo JL: Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity. Mol Med Rep 12: 5737-5745, 2015.
APA
Hogg, T., Mendel, J.T., & Lavezo, J.L. (2015). Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity. Molecular Medicine Reports, 12, 5737-5745. https://doi.org/10.3892/mmr.2015.4146
MLA
Hogg, T., Mendel, J. T., Lavezo, J. L."Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity". Molecular Medicine Reports 12.4 (2015): 5737-5745.
Chicago
Hogg, T., Mendel, J. T., Lavezo, J. L."Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity". Molecular Medicine Reports 12, no. 4 (2015): 5737-5745. https://doi.org/10.3892/mmr.2015.4146
Copy and paste a formatted citation
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Spandidos Publications style
Hogg T, Mendel JT and Lavezo JL: Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity. Mol Med Rep 12: 5737-5745, 2015.
APA
Hogg, T., Mendel, J.T., & Lavezo, J.L. (2015). Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity. Molecular Medicine Reports, 12, 5737-5745. https://doi.org/10.3892/mmr.2015.4146
MLA
Hogg, T., Mendel, J. T., Lavezo, J. L."Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity". Molecular Medicine Reports 12.4 (2015): 5737-5745.
Chicago
Hogg, T., Mendel, J. T., Lavezo, J. L."Structural analysis of a type 1 ribosome inactivating protein reveals multiple L‑asparagine‑N‑acetyl‑D‑glucosamine monosaccharide modifications: Implications for cytotoxicity". Molecular Medicine Reports 12, no. 4 (2015): 5737-5745. https://doi.org/10.3892/mmr.2015.4146
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